中文摘要：

采用正交设计法研究适合篦子三尖杉（Cephalotaxus oliveri）的ISSR—PCR反应体系，对影响ISSR—PCR的模板、引物、Mg^2＋、dNTP、Taq酶等5个因素进行了优化。确定了篦子三尖杉ISSR—PCR扩增的最佳体系，即20μl的反应体系中包括：1μl 10×PCRbuffer、2．5mmol／L Mg^2＋、0．2mmol／L dNTPs、0．25mol／L的ISSR引物、1．5UTaqDNA聚合酶、50ng模板DNA。运用优化的体系对江西武功山篦子三尖杉总DNA进行ISSR—PCR扩增，获得较好效果。

英文摘要：

The orthogonal design was used to optimize ISSR - PCR amplification system, such as DNA template, primers, concentration of Mg^2＋ , dNTPs and Taq DNA polymerase were investigated to optimized the ISSR -PCR reaction system of Cephalotaxus oliveri. A sutable ISSR - PCR reaction system was established, namely 20 lreaction system contaning： 1 μl 10 × PCRbuffer 2.5mmol/LMg^2＋ , 0.2 mmol/L dNTPs, 0.25 μmol/L Primer, 1.5U TaqDNA polymerase, 50ngDNA. The optimized system laid the groundwork for ISSR molecular analysis of of Cephalotaxus oliveri.