水体中磷的循环与再生依赖于微生物的分解作用,因此对水体中有机磷分解细菌的研究是弄清水体磷循环的关键所在。本研究从太湖有机聚集体上分离纯化出一株能对有机磷(卵磷脂)进行降解的菌株,命名为pjj-1。从形态、生理生化、Biolog和16S rDNA序列等方面,对该有机磷降解细菌Pjj-1进行了鉴定。结果显示,其为不动杆菌属中的琼氏不动杆菌(Acinetobacter junii).该菌株与已报道琼氏不动杆菌的16S rDNA核苷酸序列有99.5%的同源性。对其降解有机磷特性的研究结果表明,该菌株在pH=7.5、温度30℃、透气性好的条件下表现出对卵磷脂有较好的降解性能。在pH中性、30℃条件下该菌株对不同形态有机磷的降解速率分别为:甘油磷酸钠(0.045mg·L^-1·d^-1)〉卵磷脂(0.019mg·L^-1·d^-1)〉微囊藻残体(0.011mg·L^-1·d^-1),表明该菌株对水体中的有机聚集体存在相当的分解能力,对小分子有机磷的降解性能更为明显。
The regeneration of phosphate depended on the decomposition of microbe in water ecosystem, so it was important to isolate and study the phosphorus-degrading bacteria in the eutrophic water. Classical physiological and biochemical methods, Biolog and 16S rDNA coding genes analysis were applied to identify the strain pjj-1 which could degrade organic phosphorus isolated from the organic aggregates of Tathu Lake. Physiological and biochemical identification showed that it belonged to A cinetobacter sp. Biolog identification showed the similarity index reached to 0.757. 16S rDNA determination and analysis was used for further identification, which indicated that the 16S rDNA sequence of pjj-lshared 99.5% homologies with that published sequence of Acinetobacterjunii from GenBank. All these results suggested that this strain belonged to one strain of Acinetobacterjunii. The most optimum degrading condition was at 30 ℃ and pH 7.0 on organic phosphorus medium, the decomposition speed characterization for different forms of organic phosphorus was: natrium-β-glyeerophospha (0.045 mg·L^-1·d^-l)〉lecithin(0.019 mg·L^-1·d^-l)〉algale dross (0.011 mg·L^-l·d^-l)respectively, which showed that pjj-1 not only could decompose the organie aggregates in the water, but had more efficient degradation ability for the simple organie phosphorus.