分别采用含5%和10%PEG-6000的Hongland营养液模拟干旱胁迫,对来自于山东省不同地区的6个野生大豆材料进行处理,研究了干旱对野生大豆幼苗叶片的花青素、叶绿素、类胡萝卜素、相对含水量,以及叶绿素荧光参数等光合作用相关生理指标的影响。结果表明:除Y1(ZYD03245,山东临朐县)外,其他5个野生大豆幼苗叶片的花青素、叶绿素和类胡萝卜素含量随着胁迫的加强均呈升高的趋势;6个材料的相对含水量及叶绿素荧光参数均随着干旱胁迫的加强呈下降的趋势。以上述指标综合评价6个野生大豆的抗旱性,Y4(山东阳谷县)和Y6(山东聊城东昌府区)最强,Y2(ZYD03269,山东无棣县)、Y3(山东垦利县)和Y5(山东广饶县)次之,Y1(ZYD03245,山东临朐县)最差。
Six wild soybeans were collected from Shandong province including Y1 ( ZYD03245, from Linqu county), Y2 (ZYD03269, from Wudi county), Y3 (from Kenli county), Y4 (from Yanggu county), Y5 (from Guangrao county)and Y6 (from Dongchangfu district). In order to investigate the drought tolerance of these wild soybeans, simulated drought treatment was carried out with PEG-6000 at 5% and 10% concentration at seedling stage. Five physiological indexes including anthocyanin content, chlorophyll content, carotenoid content, relative water content and chlorophyll fluorescence parameters were determined to assess the drought tolerance of the 6 wild soybeans. The results showed that : ( 1 ) as the simulated drought stress increased, anthoeyanin content, chlorophyll content and carotenoid content increased in all wild soybeans except Y1 ; (2) relative water content and chlorophyll fluorescence of all wild soybeans increased as simulated drought stress increased. From the point of view of the anthocyanin content and carotenoid content, the order of drought tolerance of the 6 wild soybeans were Y4 〉 Y6 〉 Y2 〉 Y5 〉 Y3 〉 Y1. On the other hand, based on relative water content and chlorophyll fluorescence, the orders of drought tol- erance were Y4 〉 Y6 〉 Y5 〉 Y1 〉 Y3 〉 Y2 and Y4 〉 Y5 〉 Y6 〉 Y3 〉 Y2 〉 Y1, respectively. Considering all the physiological indexes together,it could be concluded that drought tolerance of Y4 and Y6 were the highest, that of Y2, Y3 and Y5 were mid- dle and that of YI was the lowest.