中文摘要：

目的：采用兔腰椎间盘内显微注射120KDa Fn片段构建腰椎间盘退变动物模型，并探讨该模型的有效性和优势。方法：新西兰大白兔30只，侧前方入路前方暴露L4-6椎间盘，微量注射器于L4-6注射120kDa Fn片段作为试验组，L，注射PBS作为对照组。分别于第2、4、8、12、16周对椎间盘进行X线、MRI、大体解剖和HE、Massion、高碘酸-Schiff染色组织病理学检测。结果：大体组织观察见术后第8周开始纤维环各层间分界模糊，外侧可见瘢痕样增生，16周出现骨样组织形成。病理染色见术后4周开始纤维环胶原出现断裂等退行性变，蛋白多糖含量降低。MRI观察发现第12周髓核高信号区强度下降，16周信号基本消失。对照组无明显变化。结论：120kDa Fn片段是诱导兔腰椎间盘退变简单、确切的方法，该方法为建立腰椎间盘退变模型提供了新思路，建立了新的腰椎间盘退变动物模型。

英文摘要：

Objective： To develop a new experimental model of rabbit lumbar intervertebral disc degeneration by injecting 120 kDa Fn fragment into its discs, and to study the feasibility and advantage of this model. Methods： 30 New Zealand white rabbits were divided into two groups. The lumbar intervertebral discs were exposured by anterior approach. The 120 kDa Fn fragment was injected into L4 and L6 disc for experimental group, while PBS was injected to L5 disc for control group. X-ray irradiation, MRI, gross and histological observation for handled discs at 2,4,8,12,16 weeks after injection were measured respectively. Results： The results of gross observation showed that the border between annulus blurred at the 8th week, and bone-like tissue could be found. Histologically, degenerated change of annulus appearted at the 4th week and the content of proteoglycan of it decreased obviously. The MRI showed that, high signal area of vertebral pulp reduced at the 12th week and disappeared at the 16th week. There were no marked changes happened in control group after operation. Conclusions： 120 kDa Fn fragment could induce lumbar intervertebral disc degeneration. It is a new way to establish the experimental animal model of disc degereration.