中文摘要：

目的：探究柴胡皂苷B2对人神经母细胞瘤细胞（SH －SY5Y）过氧化氢损伤后的作用。方法采用体外细胞培养的方法，建立 SH －SY5Y神经细胞 H2 O2损伤模型。分别将含体积分数0.05、0．10、0．20柴胡皂苷B2（10^－4 mol／L）的小牛血清按10％的比例加入SH －SY5Y细胞中，然后采用140μmol／L的H2 O2进行处理，平行设立10％小牛血清组和空白血清未经H2 O2处理组作为模型组和空白组。观察各组细胞形态，同时测定细胞存活率，检测柴胡皂苷B2作用下SH －SY5Y神经细胞的生存状态。结果同模型组比较，1×10^－6 mol／L和2×10^－6 mol／L的柴胡皂苷B2均能显著减轻 H2 O2造成的S H －SY5Y神经细胞的过氧损伤，明显提高细胞的存活率。结论柴胡皂苷B2对过氧化氢诱导损伤的人神经母细胞瘤细胞具有明显的保护作用。

英文摘要：

Objective To investigate the effect of Saikosaponin B2 on the damage of cultured SH -SY5Y cells. Methods Damaged SH -SY5Y cells model was established with the method of cell culture in vitro. 10% calf serum including volume fraction 0. 05 ,0. 10 ,0. 20 Saikosaponin B2 （10^-4 mol/L） were added respectively into the SH -SY5Y cells ,which were then treated with 140μmol/L hydrogen perox‐ide （H2 O2 ）. 10% calf serum group and blank serum without H2 O2 -treated group were as the model group and the control group. The effect of Saikosaponin B2 was observed by morphological identification , colorimetric MTT assay. Results Both Saikosaponin B2 of 1 × 10^-6 mol/L and 2 × 10^-6 mol/L can relieve the damage of SH -SY5Y cells and increase the survival of the cells. Conclusions Saikosaponin B2 can protect the cultured SH -SY5Y cells from damage induced by H2 O2.