中文摘要：

目的：研究P35（L—ficolin）作为一种凝集素补体，能否结合HCV包膜糖蛋白E1。方法：用PCDNA3-P35质粒转染细胞，建立稳定表达P35的细胞系CT26-P35，RT-PCR和Western Blot检测该细胞系，建立稳定表达HCVE1蛋白的细胞系E1-CT26，用FCM检测该细胞胞内胞膜上E1的表达。用FCM检测蛋白P35与HCVE1的作用。结果：成功建立稳定细胞系P35-CT26。E1-CT26细胞胞内和胞膜均有E1表达。FCM分析，P35与E1-CT26结合能力强于P35与CT26的结合。结论：成功建立稳定表达P35的细胞系；P35能识别并结合HCV的糖蛋白E1。

英文摘要：

Objective： To study whether P35 can bind to HCV E1 as a kind of C-type lectin. Methods.. The vector PCDNA3-P35 was constructed and transfect it into CT26 cells. RT-PCR and Western Blot were used to identify the P35-CT26 cell line. And a stable cell line, E1-CT26, was constructed before the E1 and the P35＇s binding ability to HCV E1 was determined by FCM. Results： The stable cell line P35-CT26 had been successfully constructed. HCV E1 could express both in the cytoplasm and on the surface of E1-CT26. FCM revealed that P35 could bind to HCV E1 more tightly than CT26 does. Conclusion： The stable cell line P35-CT26 had been successfully constructed and P35 could recognize and bind to HCV El.