中文摘要：

提取经条斑病菌（Xanthomonas oryzae pv．oryzicola）侵染诱导后的水稻mRNA，利用SMART^TM技术于载体pGADT7-SfiAB上构建了水稻cDNA文库。检测发现，文库中含有水稻病程相关蛋白基因OsPR—1a、OsPR—1b和PAL。随机提取cDNA文库克隆的质粒，酶切分析发现，该cDNA文库的插入片段分布在500～2000bp之间，平均大小约为1kb，重组率达95％。上述结果表明，水稻受条斑病菌侵染的cDNA文库质量较好，这为研究条斑病菌致病性因子与水稻互作机制奠定了工作基础。

英文摘要：

Constructing a rice cDNA library is a fundamental basis for understanding molecular interactions of rice with Xanthomonas oryzae pv. oryzicola, the critical pathogen of bacterial leaf streak in rice. The total RNA were extracted from rice leaves previously infected for 10 hours by the pathogen. The cDNA were reversely transcripted from the mRNA using SMARTTM technique and then ligated into a vector pGADT7-SfiAB at the sites of SfiA and SfiB. Collections of the ligated plasmid DNA were the cDNA library of rice which had interactions with the pathogen. The PCR amplification of OsPR-1a, OsPR-1b and PAL as pathogenesis-related protein genes indicated that the cDNA library contained those genes. Digestion of randomly selected clones of the library with the enzyme Sfi Ⅰ demonstrated that the sizes of inserted fragments in the vector were between 500 -2 000 bp, that the average size was about 1 kb, and that 95% of the library clones were recombinants. The above suggests that the constructed cDNA library of rice is of good quality and of use for isolation of interacting proteins with pathogenicity determinants of the bacterium by a yeast twohybrid system.