中文摘要：

肽核酸（Peptide Nucleic Acid，PNA）是近十几年发展起来的以中性酰胺键为骨架的脱氧核糖核酸（Deoxyribonucleic Acid，DNA）类似物，其结构介于多肽和DNA之间。由于PNA能够与DNA和RNA特异性地结合，可以制备PNA探针。与DNA探针相比，其杂交的稳定性和特异性增加且能在低盐浓度下进行杂交。本文从DNA和PNA的分子结构和性质、DNA探针和PNA探针的设计制备、杂交亲和性、杂交动力学以及在生物传感器上的应用等方面进行了系统比较。

英文摘要：

Peptide nucleic acid （PNA） with peptide linkages as its backbone is a brand new kind of compounds which are developed in recent years. It is an analog of DNA in molecular structure. PNA can hybridize DNA and RNA specifically, thus it can be used as probes. Compared with traditional DNA probes, PNA probes enjoy higher hybrid-stabilization, better specificity, and it can hybridize target substance under low salt ion concentration. This paper makes a systemic comparison between PNA probe and DNA probe in the aspects of probes preparation, hybridization specificity, hybridization kinetics and biosensors applications.