中文摘要：

目的 探讨DENND1B基因多态性在儿童哮喘患者中的分布及其与患者血清IgE水平的关系.方法 选取哮喘儿童296例（哮喘组）,同期健康体检儿童225例（健康对照组）,同时提取外周血基因组DNA,通过高温连接酶检测法（PCR-LDR）检测DENND1B基因rs1747815 、rs1775444、rs1775456位点的基因型.采用免疫散射比浊法检测样本血清总IgE水平.结果 rs1747815位点3组基因型（GG/GA/AA）及rs1775456位点3组基因型（AA/GA/GG）在健康对照组与哮喘组分布频率差异无统计学意义（P〉0.05）.rs1775444位点3组基因型（CC/TC/TT）在健康对照组与哮喘组分布频率差异有统计学意义（P〈0.05）,其中,哮喘组3种基因型患者中血清IgE水平差异均无统计学意义（P〉0.05）.结论 DENND1B基因多态性与儿童哮喘有一定相关性,但与哮喘患者血清IgE水平无相关性.

英文摘要：

Objective To investigate the association between DENND1B gene polymorphisms and childhood asthma. Methods Blood samples of 296 children with asthma and 225 children without history of allergic diseases were collected. Genomic DNA were extracted from all blood samples,PCR LDR reaction was employed to detect the rs1747815,rs1775444 and rs1775456 mutation in DENND1B gene. Genotype and allele distribution were analyzed. Meantime, total serum IgE levels were detected in patients with asthma in different genotypes. Results There was no significant difference in genotype and genotypic distribution of SNP loci rs1747815 and rs1775456 between asthma group and control group （P〉0.05）. However,significant difference in genotypic distribution of rs1775444 between these two groups was detected（P〈0.05）. In asthma group, genotype and genotypic distribution of SNP loci rs1775444 were not related with total serum IgE levels（P〉0.05）. Conclusion rs1775444CT genotype is significantly associated with childhood asthma,but not associated with total serum IgE levels.