中文摘要：

应用高速逆流色谱法（HSCCC）分离纯化蔓荆子中的活性成分。以石油醚-乙酸乙酯-甲醇-水（体积比为3：6：3.6：3）为两相溶剂体系,在转速为800r/min、流速为1.5mL/min、检测波长为254nm的条件下进行分离,所得馏分经高效液相色谱法（HPLC）检测,并经电喷雾电离（ESI）质谱和核磁共振谱（NMR）鉴定化合物的结构。从250mg蔓荆子粗提物中一次性分离得到4个化合物,分别为23mg对羟基苯甲酸、15mg3,6,7-三甲基槲皮万寿菊素、24mg蔓荆子黄素和5mg蒿黄素,其纯度约为93.1%、97.3%、98.7%和98.5%。该法具有简便、快速、重复性好的优点,为分离蔓荆子中的活性成分提供了新的方法。

英文摘要：

Vitex trifolia L.var.simplicifolia Cham.is widely distributed in Asia,and its fruits are used as a folk medicine for headaches,colds,migraine,eyepain,etc.In order to effectively separate high-purity active components from the seeds of Vitex trifolia L.var.simplicifolia Cham.,a high-speed counter-current chromatography（HSCCC）procedure was performed to separate four components from the crude extract of the fruits.A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water（3：6：3.6：3,v/v/v/v）was used.Within 230 min,23 mg of 4-hydroxybenzoic acid,15 mg of 3,6,7-trimethylquercetagetin,24 mg of casticin and 5 mg of artemetin were obtained from250 mg of the crude extract of Viticis Fructus in one-step elution under the conditions of a flow rate of 1.5 mL/min,800 r/min and the detection wavelength of 254 nm.The purities of the four fractions were 93.1%,97.3%,98.7%and 98.5%,respectively.The obtained fractions were analyzed by high performance liquid chromatography（HPLC）,and identified by electrospray ionization mass spectrometry（ESI-MS）,1 H-nuclear magnetic resonance（NMR）and 13C-NMR.The results indicate that HSCCC is a powerful technique for the purification of active components from Viticis Fructus.