中文摘要：

目的探讨外周血造血干细胞（PBSC）动员采集的方法及其效果。方法对恶性血液病（多发性骨髓瘤、急性白血病和淋巴瘤）患者及健康供者经单一粒细胞集落刺激因子（G-CSF）和化疗联合粒细胞集落刺激因子方案动员后,使用血细胞分离机采集外周血造血干细胞,分析不同动员方案、疾病、年龄、性别及供者的动员采集。结果所有患者和健康供者均成功动员和采集到了PBSC（MNC〉5×108/kg,CD34＋〉2×106/kg）;健康供者的动员效果为MNC（8.25±3.07）×109/L;恶性血液病患者中,急性髓细胞白血病患者采集所获得的MNC最多,为（7.48±2.62）×108/kg,多发性骨髓瘤患者最差,为（5.06±1.50）×108/kg;急性淋巴细胞白血病患者采集所获得的CD34＋最多,为（5.36±2.64）×106/kg,多发性骨髓瘤患者最少,为（3.45±0.76）×106/kg。化疗联合G-CSF和IL-11为最好的动员方案;18—60岁患者采集效果最好,60—65岁患者最差;男性较女性好。结论健康供者较恶性血液病患者动员效果好,健康供者中性别对采集效果无影响;无论是健康供者还是恶性血液病患者,动员、采集过程副作用小。

英文摘要：

Objective To investigate the factors influencing the efficiency of mobilization and collection of peripheral hematopoietic stem cells.Methods After mobilization with granulocyte colony stimulating factor（G-CSF）or with combination of G-CSF and chemotheraphy for patients of hematologic malignancies and heathy donors,the peripheral blood hematopoietic stem cells were collected by CS 3000 PLUS blood cell separator.The effect of different diseases,ways of mobilization,age,and sex on the mobilization and collection was analyzed.Results The collection was successful with all cases,with MNC5×108/kg,and CD34＋2×106/kg.An average of（8.25±3.07）×109/L mononuclear cells（MNCs）were collected from heathy donors.In patients with malignant hemotologic disorders,the MNCs collected form ANLL were（7.48±2.62）×108/kg,from patients with multiple myeloma were（5.06±1.50）×108/kg;and the collection of CD34＋ cells was the best[（5.36±2.64）×106/kg]in acute lymphoblastic leukemia,and worst with multiple myeloma[（3.45±0.76）×106/kg].The best protocol of mobilization was combination of chemotheraphy with G-SCF and IL-11.The collection is more efficient in age group between 18-60 years;and in men better than in women.Conclusion The result of mobilization for heath donors is better than that for patients of hematologic malignancy.There is no difference in collection between male and female.In the mobilization and collection process,there are only minor side effects in healthy donors and patients with malignant haematologic disorders.