中文摘要：

以热纤维梭菌（Clostridium thermocellum LQRI）和嗜热厌氧乙醇菌（Thermoanaerobacter ethanolicus X514和Thermoanaerobacter pseudoethanolicus 39E）为对象,以不同浓度纤维素Avicel为底物,研究嗜热厌氧乙醇菌加入LQRI纯培养体系后对纤维素酒精浓度和终产物分布的影响.结果表明,加入X514或39E后培养液中乳酸盐浓度急剧上升,后者乳酸盐浓度明显高于前者（＊＊p〈0.01,差异显著）,其最高值分别约为15.5mmol·L-1和13.8mmol·L-1,而乙酸盐浓度的变化特征与对照没有显著差异,总体呈下降趋势;对照和加入39E后培养液中还原性糖浓度一直呈上升趋势,其最高值分别约为2837.5μg·mL-1和2254.1μg·mL-1,而加入X514后培养液中还原性糖浓度急剧下降,其最低值约为466.7μg·mL-1;加入X514或39E后,培养液的酒精浓度就明显高于对照（＊＊p〈0.01）,此后加入X514的处理培养液酒精浓度呈急剧上升趋势,其增加幅度明显高于加入39E的处理,其最高值分别约为64.1mmol·L-1和20.0mmol·L-1.此外,分别以1%和2%Avicel为底物进行试验,其实验结果和趋势基本相同,进一步证实了在LQRI纯培养体系中加入X514或39E后会促进体系的发酵作用.X514不仅可以利用六碳糖,而且还可以发酵五碳糖生产酒精,提高体系酒精的生产能力.

英文摘要：

Ethanol production with different concentrations of cellulose Avicel as the substrate was evaluated in a batch system using a mono-culture of a cellulolytic ethanol producing strain （Clostridium thermocellum strain LQRI） and specific co-cultures of LQRI in combination with one of the the non-cellulolytic ethanol producing strains （Thermoanaerobacter ethanolicus strain X514 or Thermoanaerobacter ethanolicus 39E） which was added to the LQRI mono-culture after six days.Results showed that lactate concentration,which was significantly higher in the specific co-culture of LQRI＋39E than of LQRI＋X514 （p0.01）,dramatically increased after the non-cellulolytic ethanol producing strain was added to the LQRI mono-culture,while no significant differences were observed in the rate of decrease of acetate concentration between the specific co-cultures and the controls.Reducing sugar concentration increased in the specific co-culture of LQRI＋39E and the control,while it decreased in the specific co-culture of LQRI＋X514.The maximum concentrations of reducing sugar in the specific co-culture of LQRI＋39E and the control were 2837.5 μg·mL-1 and 2254.1 μg·mL-1 respectively,while theminimum concentration in the specific co-culture of LQRI＋X514 was 466.7 μg·mL-1.Most importantly,ethanol concentration in the specific co-cultures was significantly higher than that in the control.The ethanol concentration in the specific co-culture of LQRI＋X514 increased dramatically after X514 was added to the mono-culture after the 6th day,and the rate of ethanol increase in LQRI＋X514 was higher than that in LQRI＋39E;the maximum ethanol concentrations were 64.1 mmol·L-1 and 20.0 mmol·L-1 respectively.Additionally,the same results were found for different concentrations of cellulose,with 1% and 2% Avicel as the substrate,which suggested further that the ethanol production abilities were promoted in LQRI mono-culture where X514 or 39E was added after six days.X514 can not only utilize hexose,but also ferment pentose to