居室环境质量的优劣与人类健康密切相关.成年人约80%~90%的时间是在室内度过,而且现代人们的生活越来越离不开空调,由于人体、房间和空调机在室内会形成了一个封闭的循环系统,容易使细菌、病毒、霉菌等微生物大量繁衍.因此,百货商场、学校教室、办公室和现代住宅等近年来成为了室内环境空气质量的研究热点.在北京市5个方向(东南西北中)共选取31户具有小孩的家庭于2009年11月~2010年10月进行试验,研究了城市居家环境空气真菌的群落结构特征.结果表明,从分离的225株空气真菌中共鉴定出24属,从鉴定出现的次数来看,其优势真菌属依次为青霉属(Penicillium)、枝孢属(Cladosporium)、曲霉属(Aspergillus)、链格孢属(Alternaria)和茎点霉属(Phoma),其中青霉属出现的次数占分离菌株总数的36%.居家环境中青霉属、枝孢属、曲霉属、链格孢属、丛梗孢属(Monilia)的出现频率较高,其中青霉属的出现频率在90%以上;浓度百分比较高的真菌依次为青霉属、枝孢属、曲霉属、无孢菌和链格孢属,它们浓度总和约占总浓度的65.0%及以上,其中青霉属浓度百分比为32.2%.在北京市取样的31户家庭中,空气真菌浓度范围为62~3 498 CFU.m^-3,平均值为837 CFU.m^-3.空气真菌总浓度与枝孢属、曲霉属、链格孢属浓度的季节变化特征基本一致,夏季明显高于春季、秋季和冬季(P〈0.05*),冬季浓度最低,而青霉属浓度的季节变化则表现为春季较高,夏季、秋季和冬季没有显著差异(P〉0.05*).北京市居家环境空气真菌浓度男孩家庭明显高于女孩家庭(P〈0.05*),并且家庭空气真菌浓度与人均居住面积呈显著负相关.
Indoor environmental quality has significant effects on human health.It is reported that adults in China spent about 80%-90% of their time in indoor environments,and a number of physically handicapped people such as the elderly and infants stayed in the room even up to 95% of their total time.Moreover,air conditioner in indoor environments becomes more and more important in modern life,and a closed circulatory system can be formed among human body,room and air conditioner in indoor environments with an air conditioner,which can make the microbes such as bacteria,viruses and mold indoors propagate rapidly or abundantly.Therefore,studies on the microbial pollution in the air at places such as mall,classroom,office,and family home have been the research hotspots recently.In the present study,the community composition and concentration variation pattern of airborne fungi were investigated from Nov 2009 to Oct 2010 in 31 family homes with children in Beijing.Results showed that 24 generas of airborne fungi in family homes were identified from 225 isolates.The most common fungi were Penicillium,Cladosporium,Aspergillus,Alternaria and Phoma.The frequency of Penicillium,Cladosporium,Aspergillus,Alternaria and Monilia was much higher than those of other fungal genera in family home,and the frequency of Penicillium was more than 90%.As for the concentration percentage,airborne fungi with most high concentrations were Penicillium,Cladosporium,Aspergillus,No-sporing,and Alternaria,and totally accounted for more than 65.0%.Penicillium contributed to 32.2% of the total airborne fungi in family homes.In the 31 family homes selected,the fungal concentration in the air ranged from 62-3 498 CFU·m^-3,and the mean concentration was 837 CFU·m^-3.Seasonal variation pattern of total fungi,and Cladosporium,Aspergillus,Alternaria concentration was consistent,and the highest fungal concentration was observed in summer,followed by spring and autumn,and the lowest in winter.Concerning the Penicillium concentration,the seasonal variation p