中文摘要：

目的 研究小白菊内酯对膀胱癌T24细胞增殖和凋亡的影响及其可能存在机制.方法 应用不同浓度的小白菊内酯作用于T24细胞,使用MTT法观察T24细胞在增殖方面的变化,流式细胞术检测小白菊内酯对细胞周期和细胞凋亡的影响,蛋白印迹法检测NF-κ B信号通路和Bcl-2家族蛋白的变化.结果 小白菊内酯对T24细胞增殖具有明显抑制作用,并呈显著浓度依赖性和时间依赖性.小白菊内酯可以诱导T24细胞发生凋亡,并可以改变T24细胞在不同细胞周期的分布,包括G1期细胞所占百分比升高,S期细胞所占百分比降低,而G2/M期细胞所占百分比无明显改变.随着小白菊内酯作用浓度的增加,T24细胞中磷酸化IκBa的表达逐渐降低,但不影响l κ Ba的表达.同时,小白菊内酯能抑制Bcl-2蛋白的表达而促进Bax蛋白的表达.结论 小白菊内酯能显著抑制T24细胞增殖,诱导T24细胞发生凋亡,其作用机制与可能与抑制T24细胞中NF-κ B信号通路的激活,调节Bcl-2家族蛋白表达变化有关.

英文摘要：

Objective To explore the effects of parthenolide on growth inhibition, cell apoptosis and corresponding molecular mechanism of human T24 bladder cancer cells. Methods The inhibitory effect of parthenolide on human T24 cells was determined by MTT assay. The cell apoptosis and the cell cycle were analyzed by flow cytometry. Alterations in signal pathway of NF- K B and Bcl-2 family proteins were analyzed by Western blot. Results Parthenolide inhibited the proliferation of T24 cells in a dose-dependent and time-dependent manner. As showed by flow cytometry, parthenolide treatment of the T24 cells led to significant Gl-phase cell cycle arrest in a dose-dependent manner and a decrease in cell population in the S-phase, whereas the population of ceils in G2/M phase did not chang significantly. Flow cytometry also showed a dose dependent increase in T24 cells apoptosis by parthenolide treatment. Western blot analysis indicated a down-regulation of phosphorylated I K Bct in a dose-dependent manner without an effect on I kBα expression in T24 cells by parthenolide treatment. Parthenolide treatment to T24 cell lines resulted a significant reduction in Bcl-2 protein expression but an increase in Bax protein expression in a dose-dependent manner. Conclusions Parthenolide treatment could lead to a significant dose-dependent and time-dependent inhibition in the growth and an increase in apoptosis of human T24 bladder cancer cells. The mechanism of action of parthenolide may be related to inhibition of NF-kB activation and regulation of Bcl-2 family proteins expression in human T24 bladder cancer cells.