中文摘要：

根据Gen Bank中a MPV-C P基因序列,设计出一对特异性引物和Taqman探针,建立了a MPV-CTaqman探针荧光定量PCR方法。对该反应体系进行优化,进行特异性、敏感性及重复性试验,并且建立标准曲线。结果表明,该方法只对a MPV-C检测为阳性,具有良好的特异性;能够检测到（3.63×102）拷贝数,具有良好的敏感性;建立的标准曲线斜率为-3.312,截距为44.66,相关系数为R2=0.999,循环阈值和模板拷贝数具有良好的相关性,组内及组间重复性好。采用a MPV-C Taqman探针荧光定量方法、普通PCR方法对来自广东、浙江地区25份番鸭疑似阳性a MPV-C的病料进行检测,其中前者23份阳性,后者18份阳性。这表明a MPV-C Taqman荧光定量PCR方法对样品的检测具有特异性和敏感性高的特点,更适合临床样品的检测。

英文摘要：

In order to establish a method of aMPV-C Taqman probe fluorescence quantitative PCR, a pair of specific primers and Taqman probe were designed based on the P gene sequences of aMPV-C in the Genbank. Then the method system was optimized by the specific, sensitive tests, and replicative experiments, and the standard curve was established. The results indicated that the system had good specificity and sensitivity, and the aMPV-C samples including 3.63 × 102 copies/μL was positively tested. The cycle threshold and template copy number also had good correlation in the standard curve. It also had repeatability within the group and between the groups. 25 positive aMPV-C samples were tested by two kinds of PCRs, and the results showed that 23 samples were detected to be positive by the established PCR, and 18 samples were detected positive by the other PCR method. Our results suggest that the aMPV-c Taqman PCR is specific and sensitive for detecting clinical samples.