中文摘要：

目的：探索体外诱导DCs活化的方法和制备肾癌树突状细胞（DCs）瘤苗。方法：制备肾癌细胞冻融抗原；取健康人新鲜血分离外周血单个核细胞（PBMC），应用GM—CSF＋IL-4刺激，诱导PBMC为iDCs,然后进行分组，采用不同因子刺激iDCs转化为mDCs，其中A组：冻融抗原负载；B组：TNF-α＋冻融抗原负载；C组：IL-1β＋冻融抗原负载；D组：TNF-α＋IL-1β＋冻融抗原负载。结果：各组均可诱导iDCs的成熟，并高表达CD86、CD80和HLA—DR；相对于其它组，D组DCs更显著上调CD83和CD54表达（P〈0．05）和IL-12分泌（P〈0.01），且D组mDCs更有效地刺激淋巴细胞增殖（P〈0．05）。结论：TNF-α＋IL-1β与肾癌细胞冻融抗原协同可有效促进DCs成熟、增强诱导淋巴细胞活化的能力。

英文摘要：

AIM： To investigate the effects of renal tumor cell lysates and other cytokines on the activation of immature dendritic cells （ iDCs ）, and to develop DCs vaccines for stimulation of renal tumour - specific immunity. METHODS： DCs induced from peripheral blood mononuclear cells of healthy volunteers were cultured and propagated in vitro using rhGM - CSF and rhIL - 4, and then were cocultured with renal tumor cell lysates and different cytokines. A group ： only with renal tumor cell lysates; B group： with renal tumor cell lysates and TNF -α; C group： with renal tumor cell lysates and IL - 1β ; D group ： renal tumor cell lysates and TNF - α ＋ IL - 1β. RESULTS ： iDCs were induced to mature in all four groups, and high level expressions of CD86, CD80 and HLA - DR were observed. Compared to other groups, DCs in D group expressed CD83 and CD54 at higher level （ P 〈 0. 05 ）, secreted higher quantity of IL - 12 （ P 〈 0. 01 ）. Moreover, mDCs in D group induced multiplication of lymphopoiesis more effectively （P 〈 0. 05 ）. CONCLUSION： Renal tumor cell lysates and TNF - α ＋ IL-1β induce the mature phenotype and IL - 12 production in DCs and synergistically promote the stimulatory effects of lymphopoiesis.