中文摘要：

目的：探讨肝细胞肝癌患者血清microRNA-224水平变化及其临床诊断意义。方法：采用实时荧光定量PCR检测42例肝细胞肝癌患者（hepatocellular carcinoma，HCC）、36例肝硬化患者（LC）、55例慢性乙型肝炎患者（CHB）以及40例健康体检者（NC）血清miR-224表达量。计算miR-224相对表达量。通过分析受试者工作特征曲线（ROC）判断miR-224表达水平在肝癌诊断中的灵敏度和特异度。结果：HCC组患者血清miR-224相对表达量均高于CHB组、LC组和NC组，差异有统计学差异（P〈0.05或P〈0.01）。HCC患者血清miR-224相对表达量与甲胎蛋白（AFP）呈正相关（P〈0.05）。与肿瘤大小、TNM分期、肿瘤分化程度以及淋巴结转移无相关性（P〈0.05）。ROC分析确定了最佳的miR-224相对表达量的临界值为3.47，灵敏度被确定为82.2％，特异性为92.8％，曲线下面积（AUC）为0.935。结论：肝细胞肝癌患者血清中miR-224特异性高表达，将使其可能成为一种新的血清学指标用于肝细胞肝癌的诊断。

英文摘要：

Objective：To investigate the serum level of miR-224 in hepatocellular carcinoma （HCC） patients and its clinical diag-nostic significance. Methods：The serum level of miR-224 was detected by real-time quantitative PCR. This study included 42 cases of patients with HCC, 36 patients with liver cirrhosis （LC）, 55 patients with chronic hepatitis B （CHB）, and 40 healthy persons （NC）. The relative expressions of miR-224 were calculated. The receiver operating characteristic （ROC） curves were analyzed to determine the sensitivity and specificity of miR-224 expression levels in HCC diagnosis. Results：Result shows that the relative miR-224 expression was higher in the serum of HCC patients than that in the CHB, LC, and NC groups. The difference was statistically significant （P〈0.05 or P〈0.01）. The relative miR-224 expression in the serum of HCC patients and alpha fetoprotein （AFP） were positively correlated （P〈0.05）. By comparison, the tumor size, TNM stage, tumor differentiation, and lymph node metastasis were not correlated with mirR-224 expression （P〈0.05）. ROC analysis shows that the best critical value of the relative expression levels of miR-224 was 3.47, with sensi-tivity of 82.2%, specificity of 92.8%, and area under the curve of 0.935. Conclusion：The serum level of miR-224 in HCC patients has high specificity, and miR-224 has great potential to become a new serological marker for the diagnosis of HCC.