中文摘要：

目的：观察代谢型谷氨酸受体（mGluRs）激动药3，5-二羟基苯甘氨酸（DHPG）对大鼠海马CAl区穿通通路（PP通路）诱发长时程抑制的作用及机制。方法：制作Wistar大鼠海马组织脑片，分为DHPG组、DHPG＋N-甲基-D-天冬氨酸（NMDA）受体拮抗药AP5组和DHPG＋双脉冲刺激（PPR）组（n分别为8、9、8），各组加入相应药物及操作后采用电生理记录加药前、后的场电位的标准化后均值。结果：DHPG组、DHPG＋AP5组、PPR组加药前、后场电位均值分别为1．0049±0．101、0．7904±0．02197（n=8，t=15，P〈0．01），1．0224±0．0724、0．828±0．01475（n=9，t=15，P〈0．01），0．9525±0．1691、1．0511±0．2934（n=8，t=5，P〈0．01）。结论：DHPG可诱发大鼠海马长时程抑制，且其属于mGluRs依赖型而非NMDA受体依赖型，并通过突触后诱发所致。

英文摘要：

OBJECTIVE： To investigate the mechanism of the inductive effect of metabotropic glutamate receptors （GluRs） agonist DHPG on long- term depression in hippocampal CA1 perforant pathway of rats. METHODS： Hippocampal tissue slices of Wistar rats were prepared and divided into three groups： DHPG group （n ： 8）, DHPG ＋ NMDA receptor antagonist AP5 group （n ~ 9）, and DHPG ＋ paired-pulse stimulation （PPR） group （n ： 8）. The standardized mean values of the field potential before and after drug treatment were recorded with the use of electrophysiological technology. RESULTS： The mean values of the field potential before vs. after drug treatment in the three groups were 1.004 9± 0.101 vs. 0.790 4 ± 0.02197（n=8, t =15, P〈0.01）, 1.0224±0.0724vs. 0.828±0.01475（n=9, t ：15, P〈0.01）, and0.9525±0.1691 vs. 1.051 1± 0.293 4 （n = 8, t= 5, P〈0.01）, respectively. CONCLUSION： DHPG can induce hippocampal long - term depression in mGluRs- dependent manner rather than NMDA receptor- dependent manner via a postsynaptic pathway.