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真菌毒素产生菌的分子鉴定研究进展
  • 期刊名称:中国农业科技导报
  • 时间:0
  • 页码:42-50
  • 语言:中文
  • 分类:TQ929.2[轻工技术与工程—发酵工程;化学工程] Q949.327.1[生物学—植物学]
  • 作者机构:[1]School of Marine Science and Technology, Harbin Institute of Technology at Weihai Weihai 264209, China, [2]Key Laboratory of Marine Bio-active Substances, State Oceanic Administration, Qingdao 266061, China
  • 相关基金:Supported by the China Ocean Mineral Resources Research and Development Association Project (No. DY 125-15-R-01) and the National Natural Science Foundation of China (Nos. 30870003, 31100090)
  • 相关项目:抑制AF污染的花生荚果际生防细菌的高通量筛选与多样性研究
作者: 王琢|闫培生|
中文摘要:

海洋的放射菌类的 36 紧张在曲霉属菌 parasiticus 和使用双文化板试金和琼脂散开方法的 norsolorinic 酸的生产的生长上从从黄海镇定、以他们的禁止的活动评估的海洋的沉积的一件样品被孤立。在他们之中,三紧张显示出强壮的抗真菌的活动并且随后作为 Streptomyces sp 被识别。由定序分析的 16S rRNA 基因。从 MA01 紧张的发酵的上层清液与氯仿和乙醇醋酸盐顺序被提取,并且摘录的活动被尖端文化试金决定。当他们的集中增加了,两个都提取禁止的菌丝体生长和毒素生产的试金结果表演,和摘录的禁止的活动增加了。这研究的结果建议海洋的放射菌类为 mycotoxins 的控制是生物学上重要的,并且这些细菌能对 mycotoxins 被用作新奇 biopesticides。

英文摘要:

Thirty-six strains of marine actinomycetes were isolated from a sample of marine sediment collected from the Yellow Sea and evaluated in terms of their inhibitory activity on the growth of Aspergillus parasiticus and the production of norsolorinic acid using dual culture plate assay and agar diffusion methods. Among them, three strains showed strong antifungal activity and were subsequently identified as Streptomyces sp. by 16S rRNA gene sequencing analysis. The supernatant from the fermentation of the MA01 strain was extracted sequentially with chloroform and ethyl acetate, and the activities of the extracts were determined by tip culture assay. The assay results show that both extracts inhibited mycelium growth and toxin production, and the inhibitory activities of the extracts increased as their concentrations increased. The results of this study suggest that marine actinomycetes are biologically important for the control of mycotoxins, and that these bacteria could be used as novel biopesticides against mycotoxins.

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