中文摘要：

为明确沃尔巴克氏体（Wolbachia）在赤拟谷盗和杂拟谷盗体内的感染状况及种间水平和垂直传播情况,首先通过PCR扩增分离细胞分裂蛋白基因（fts Z）目的片段方法检测这2个近缘种拟谷盗体内是否存在Wolbachia,并将同性别或异性别的赤拟谷盗和杂拟谷盗混合饲养40 d,然后测定赤拟谷盗及其与杂拟谷盗的正反交杂种后代体内Wolbachia的感染状况。结果显示,杂拟谷盗体内存在Wolbachia,而赤拟谷盗体内不存在该菌;混合饲养后,赤拟谷盗及其与杂拟谷盗的正反杂交后代体内也均没有检测到Wolbachia的存在,未发现Wolbachia在杂拟谷盗和赤拟谷盗种间的水平和垂直传播。

英文摘要：

To define the infection and interspecifically horizontal and vertical transmission of Wolbachia in two close- ly related flour beetles Tribolium castaneum （Herbst） and T. confusum （Jac. du Val. ）, Wolbachia was detected in both beetle species by PCR amplification offisZ target gene fragment. After same-sex or opposite-sex mixed feeding, the infec- tion of Wolbachia in the red flour beetles T. castaneum and the reciprocal hybrids of T. castarteum and T. confusum was ex- amined. The wolbachia infected confused flour beetles T. confusum exclusively. The red flour beetles T. castaneum and the reciprocal hybrids were Wolbachia-free. These suggests that interspecifically horizontal and vertical transmissions of Wolba- chia did not occur in the two closely related flour beetles T. castaneum and T. confusum, and T. castaneum may have an internal mechanism inhibiting the reproduction and survival of Wolbachia.