中文摘要：

目的研究转录因子Id1在小鼠颈动脉损伤后血管壁的动态表达及在损伤血管新生内膜增生中的作用。方法采用昆明小鼠颈动脉球囊损伤动物模型,随机均分为四组：正常对照组、血管损伤后7天、14天及28天组。HE染色评价血管内膜增生情况。逆转录聚合酶链反应、Western blotting及免疫组织化学染色等方法检测血管壁Id1的表达。结果正常对照组小鼠颈动脉内膜/中膜面积比值很小,损伤7天组颈动脉内膜/中膜面积比值显著高于正常对照组（P〈0.05）,损伤14天组和28天组颈动脉内膜/中膜面积比值明显高于7天组（P〈0.05）。正常对照组小鼠颈动脉血管壁Id1 mRNA和蛋白表达很低,损伤后7天血管壁Id1 mRNA和蛋白表达水平明显增加,损伤14天Id1 mRNA和蛋白表达显著高于7天组（P〈0.05）,但损伤28天组Id1 mRNA和蛋白表达较14天组有所降低（P〈0.05）。免疫组织化学显示正常血管壁Id1表达很弱;随血管损伤时间延长Id1表达逐渐增强,14天达到高峰,28天表达出现减弱。结论颈动脉损伤后局部Id1表达动态改变伴随血管损伤新生内膜增生的变化,提示转录因子Id1可能参与血管损伤后新生内膜增生的调控过程。

英文摘要：

Aim To explore the relationship between Id1 expression in neointima and vascular neointima hyperplasia. Methods The model of carotid arteries injured was established by catheter in mice. Kunming mouse were randomly divided into 4 groups：control group,groups of 7 day,14 day and 28 day after the injury. The vascular morphology was analyzed by HE dying. The expression of Id1 mRNA was measured by reverse transcriptase PCR,and the protein of Id1 was assessed by Western blotting and immunohistochemistry. Results The ratio of intima area to media area （IA/MA） was significantly increased in 7 day after vascular injury than that in control group （P〈0.05）,and further to increase on 14 day and 28 day after injury （P〈0.01）. The expression of Id1 mRNA in experimental groups was significantly increased than that in control group （P〈0.05）. The Id1 mRNA was significantly increased on 14 day than that in 7 day and 28 day after vascular injury （P〈0.05）. The expression of Id1 protein was similar with the expression of mRNA. The low level Id1 was detected in the uninjured vessel by the immunohistochemistry. However,Id1 staining was increased only in the medium at 7 days,enhanced significantly in medium and neointima at 14 days. However,Id1 staining was decreased at 28 days. Conclusion The Id1 expression was in the dynamic changes in the process of vascular neointema hyperplasia after vascular endothelial injury,and it maybe play a role in the neointema hyperplasia after vascular injury.