中文摘要：

目的研究常规给水处理工艺水中有机提取物致细胞氧化应激的影响。方法以人胚肝细胞（L02细胞）为靶细胞，水样有机提取物设1．2、6、30、150ml／ml培养液四个剂量，二甲基亚砜（DMSO，10ml／L）为溶剂对照，H2O2（100μmol／L）为阳性对照，染毒L-02细胞24h后，检测细胞内脂质过氧化产物丙二醛（MDA）、还原型谷胱甘肽（GSH）和8．羟基脱氧鸟苷（8-OHdG）的含量。结果与溶剂对照相比，给水处理各工艺环节的水样有机提取物在较高浓度（30、150ml／ml培养液）时均使L-02细胞MDA含量增加（P〈0．05，P〈0．01），GSH含量下降（P〈0．05，P〈0．01）；各工艺环节中MDA含量的变化趋势是：预氯．混凝沉淀工艺使其增加，沉淀工艺使其降低，加氯消毒工艺使MDA含量再次增加；GSH含量在上述相应工艺环节则表现为先下降，再上升，后又下降的趋势。与溶剂对照相比，各工艺环节水样有机提取物浓度为30ml／ml培养液时，L-02细胞中8-OHdG水平升高（P〈0．05，P〈0．01），且与MDA的变化趋势一致。结论两次加氯工艺增强了有机提取物对L-02细胞的氧化应激作用，使脂质过氧化反应增强、抗氧化作用降低、DNA氧化损伤增加。沉淀、过滤工艺可以减弱上述有害效应。

英文摘要：

Objective To investigate the effects of drinking water treatment procedures on the cellular oxidative stress induced by organic extracts of drinking water. Methods Human embryonic liver cell line （L-02） was treated with water organic extracts of 1.2, 6, 30 and 150 ml water samples per millililer culture. DMSO （10 ml/L） and H2O2 （100 μmol/L） were used as the negative and positive controls, respectively. MDA, GSH and 8-OHdG in L-02 cell cultures were determined after 24 h of treatment with water organic extracts. Results The contents of MDA were significantly increased and the contents of GSH were significantly decreased in L-02 cells exposed to organic extracts at the concentrations of 30 and 150 ml water samples/ml culture as compared with solvent control （P〈0.05 or P〈0.01）. Changes of MDA level showed tendencies to increase after pre-chlorination plus coagulation-sedimentation and to decrease after precipitation, then to increase again after post-chlorination. The change tendencies of GSH were contrary to those of MDA. Levels of 8-OHdG in L-02 cells exposed to organic extracts at the concentration of 30 ml/ml culture were significantly increased as compared with solvent control （P〈0.05, P〈0.01） and showed the same change tendencies of MDA. Conclusion Both the pre-chlorination and post-chlorination steps lead to the increase of oxidative stress in L-02 cells, but precipitation and filtration show the opposing effects.