中文摘要：

目的观察环维黄杨星D对正常人肝细胞（L-02）的体外细胞毒性作用。方法予以不同浓度的环维黄杨星D作用L-02细胞，MTT法检测细胞增殖情况；AnnexinV＋PI双染色流式细胞术测定细胞凋亡率；倒置显微镜观察细胞形态；用酶标仪检测乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）水平以及ATP酶活力。结果环维黄杨星D可显著抑制L-O2细胞的增殖，改变细胞形态，促进细胞凋亡和LDH释放，降低Na＋、K＋-ATP酶、Ca2＋、Mg2＋-ATP酶及Ca2＋-ATP酶活性；但对MDA和SOD无显著影响。结论一定剂量环维黄杨星D对肝细胞L-O2有损伤作用，其机制可能与抑制ATP酶活力有关。

英文摘要：

Objective To explore in vitro cell toxicity of cyclovirobuxi- hum - D on normal human hepatocyte LO2. Methods LO2 were treated with different concentrations cyclovirobuxinum - D for 12,24,48 h. The proliferation was assessed by MTT. The rate of apoptosis was detected by using FCM with Annexin V -FITC and PI double labeling method. The changes of celluar morphology was investigated by inverted microscope. LDH, MDA, SOD contents and the activities of Na ＋ , K ＋ - ATPase, Ca2 ＋ , Mg2 ＋ - ATPase, Ca2 ＋ - ATPase were determined by using enzyme mark instrument. Results Cyclovirobuxinum - D could significantly inhibit cell proliferation, cyclovirobuxinum - D changed celluar morphology. It increased significantly the rate of apoptosis and LDH level, decreased the activities of Na ＋ , K ＋ - ATPase, Ca2 ＋ , Mg2 ＋ - ATPase, Ca2 ＋ -ATPase . There were no obvious changes in the SOD, MDA content. Conclusion A certain dose of cyclovirobuxinum -D could result in the cells damage effects. Mechanism of cyclovirobuxinum - D might be related with inhibition the activities of Na ＋ , K ＋ - ATPase, Ca2 ＋ , Mg2 ＋ - AT- Pase, Ca2 ＋ - ATPase in cell membrane.