中文摘要：

目的分析上调CD99基因对经典霍奇金淋巴瘤（cHL）细胞株L428形态和免疫表型的影响，探究CD99基因对H／RS细胞发生发展的意义。方法鬼笔环肽染色和大细胞计数分析过表达CD99基因对L428细胞形态的影响；免疫细胞化学和流式细胞检测分析上调CD99基因对L428细胞免疫表型的改变。结果CD99基因的过表达导致L428细胞变小、骨架发生重建，丢失了cHL的诊断标记CD30和CDl5，重现B细胞表型PAX5、CDl9、CD79α、BCL-6和CD10。结论CD99基因过表达调控L428细胞向B细胞方向转化，提示eHL的B细胞表型的缺失很可能是CD99基因下调的结果。

英文摘要：

Objective To explore the effect of CD99 overexpression on the morphology and differentiation-related phenotypes of classical Hodgkin＇s lymphoma cell line L428 and investigate the role of CD99 gene in Hodgkin/Reed-Stemberg （HRS） cell generation and transformation. Methods The effect of CD99 overexpression on the cell morphology was detected by HE staining and phalloidm staining. Differentiation-related protein expressions were detected by immunocytochemistry and flow cytometry after stable tansfection of CD99 genem L428 cells. Results CD99 overexpression caused a decrease of the cell size and reorganization of the actin cytoskeleton in L428 cells. Upregulation of CD99 led to the loss of classical Hodgkiffs lymphoma diagnosis marker CD30 and CD15 and the restoration of the B-ceU makers of PAX5, CD19, CD79α, BCL-6, and CD10. Conclusion CD99 overexpression leads to redifferentiafion of L428 cells towards B cells, suggesting that the loss of B-cell phenotype in classical Hodgkin＇s lymphoma is very likely a result of down-regulated CD99 expression.