中文摘要：

目的检测miR-9在霍奇金淋巴瘤H／RS细胞的表达及其对靶点PRDM1的调控作用。方法采用荧光定量反转录聚合酶链反应（RT—PCR）和原位杂交方法从定量和定位两方面检测miR．9在正常CD；B淋巴细胞及8株淋巴造血系统肿瘤细胞株中的表达，并将化学合成的miR．9反义寡核苷酸瞬时转染IA28细胞使其沉默，观测PRDM1蛋白表达的变化。结果荧光定量RT—PCR结果显示L428细胞株miR．9的表达远高于正常的cDjB淋巴细胞和其他淋巴瘤细胞株f分别为OCI—Lyl细胞、Raji细胞、EB病毒（EBV）’永生化B细胞、间变性大细胞淋巴瘤（ALCL）细胞的47倍、50倍、7倍、6倍]；原位杂交结果显示miR-9的表达定位于胞质，在L428细胞呈弥漫强阳性表达。在弥漫大B细胞淋巴瘤（DLBCL）和伯基特淋巴瘤细胞株呈散在弱阳性表达，在KARPAS-299和Jurkat细胞表达阴性；瞬时下调IA28细胞中miR-9后PRDM1蛋白的表达水平升高。结论miR-9在经典霍奇金淋巴瘤中扮演着“癌基因”的角色，可能通过转录后调控PRDMl基因发挥着潜在的调节终末B细胞分化功能。

英文摘要：

Objective To explore the expression of miR-9 in H/RS cells and its regulation on target PRDM1. Methods miR-9 expression in normal CD19 B-cell subsets and eight lymphoma cell lines was detected by fluorescence quantitative RT-PCR and in situ hybridization （ISH）, for quantification and location, respectively. Chemically synthesized antisense oligonucleotide of miR-9 was transiently transfected into IA28 for its silence, and the PRDM1 expression was tested. Results Fluorescence quantitative RT-PCR showed that the expression of miR-9 in L428 cells was marked higher than that of normal CD{9 B-cell subsets and other lymphoma cell lines （the expression of miR-9 in IA28 cells was 47-fold of OCI-Lyl, 50-fold of Raji cells, 7-fold of EBV immortalized B cell line, and 6-fold of ALCL cell line）. ISH indicated that miR-9 located in cytoplasm, it was a diffuse and strong positive in IA28, scattered and weak in DLBCL and Burkitt＇ s lymphoma cell lines, while negative in KARPAS-299 or Jurkat cell lines. Transient down-regulation of miR-9 in IA28 leded to the increase of PRDM1 protein. Conclusion miR-9 plays the role of ＂cancer gene＂ in cHL, and may exert a potential function in regulating terminal B cell differentiation through a post transcription regulation of PRDM1 gene.