中文摘要：

该研究采用RT-PCR技术,从抗病的中国野生华东葡萄‘白河-35-1’和感病的欧洲葡萄‘佳丽酿’中克隆了液泡加工酶基因（γVPE）,分别命名为VpγVPE和VvCγVPE。克隆的2个γVPE基因cDNA长度均为1 624bp,ORF为1 482bp,编码493个氨基酸。氨基酸多序列对比分析发现,‘白河-35-1’、‘佳丽酿’、‘无核白’和‘黑比诺’葡萄中的γVPE基因底物结合口袋域的3个关键氨基酸之一的丝氨酸（Ser395）均变为丙氨酸（Ala）,与其他植物的VPE基因底物口袋结合域有所不同。实时荧光定量PCR表明,在白粉菌诱导后的不同时期内,γVPE基因在感病葡萄和抗病葡萄中的表达模式不同,抗病株系中VpγVPE基因的表达量在诱导后的前期（4h和48h）和后期（168h）均有所增加,而感病株系中VvCγVPE基因在诱导后4h表达量最高,随后降低。γVPE基因在白粉菌诱导后不同时期内表达量的变化,表明γVPE基因在一定程度上与葡萄的抗性相关。研究结果为进一步揭示γVPE基因在抗病过程中的分子机理奠定了基础。

英文摘要：

Vacuolar processing enzyme（γVPE）was cloned from Chinese wild Vitis pseudoreticulata accession.‘Baihe-35-1＇and Vitis vinifera cv.‘Carinena＇by RT-PCR.They were named VpγVPE and VvCγVPE.The full length of VpγVPEand VvCγVPEcDNA are 1 624 bp,and the open reading frame are1 482 bp,encoding 493 amino acid.The alignment of the amino acid sequence revealed that Ser 395,one key amino acid in substrate pocket,was replaced by Ala in Vitis pseudoreticulata accession.‘Baihe-35-1＇,Vitis vinifera cv.‘Carinena＇,Vitis vinifera cv.‘Thompson seedless＇,and Vitis vinifera cv.‘Pinot Noir＇.The different expression profile of VpγVPE gene and VvCγVPE gene in different periods after powdery mildew induced were analyzed by Real-time fluorescent quantitative PCR.After induction the expression of VpγVPEgene increase slightly in 4h,48 h,168hand the VvCγVPEgene shows predominant expression in 4hafter that it decreases slowly.The study shows thatγVPEis related to resistant to pow-dery mildew.The study provides reference value to the molecular mechanism ofγVPEgene in disease resistance.