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中文摘要：
从无核白葡萄（VitisviniferaCV．Thompsonseedless）中克隆获得一个胚珠发育相关MADS—box基因。该基因cDNA全长1248bp，ORF为729bp，编码一个含有243个氨基酸的蛋白。氨基酸多序列比对和系统发育分析显示，该基因属于SVP类MADS—box基因。采用半定量技术进行表达分析表明，该基因在葡萄的根、茎、叶、花蕾、盛花和胚珠中均有表达，但是有强弱差异。实时定量PCR技术分析表明在无核葡萄品种无核白胚珠发育过程中基因表达量先升后降，而在有核葡萄品种黑比诺胚珠发育过程中表达量一直呈下降趋势。
英文摘要：
A MADS-box gene Thompson seedless. The full-1 and encoding 243 amino acids. SVP-like MADS-box genes. T associated with ovule development was cloned from Vitis. vinifera cv. ength of cDNA gene was 1 248 bp, including a 729 bp open reading frame, The homology and phylogenetic analysis showed that this gene belonged to he gene was expressed in all of the grape tissue type examined by semi-quan- tity RT-PCR, including root, stem, leaf, bud, full bloom and ovules, but varied widely in the level of ex- pression. Real-time PCR analysis revealed that gene expression level during ovule development in Thomp- son increased firstly and then decreased. In contrast to this, gene expression level in Pinot showed down- ward trend in all stages of development.
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