中文摘要：

目的探讨血清miR-192、miR-215作为胃癌诊断标记物的可能性。方法收集2011年9月至2012年1月四川省人民医院29例术前胃癌患者和10名健康对照者血清并提取总RNA，反转录后采用实时荧光定量PCR的方法检测miR-192、miR-215相对表达量，收集胃癌患者的详细临床病理资料，比较miR-192、miR-215在胃癌患者与健康人之间的差异，并与临床病理特征之间的关系进行分析，构建受试者工作特征曲线（ROC曲线），通过曲线下面积（AUC）计算miR-192、miR-215检测及其联合应用诊断胃癌的敏感性和特异性。结果胃癌患者血清中miR-192、miR-215表达水平分别较正常对照组升高8.87倍（P＝0.002）和4.14倍（P＝0.037）。在不同肿瘤分期、淋巴结转移、肿瘤大小的胃癌患者之间比较，血清中miR-192、miR-215表达无统计学差异。 ROC曲线分析显示，miR-192、miR-215检测及其二者联合应用于胃癌诊断的AUC值分别为0.833（95％CI：0.793～0.993，P＜0.001）、0.724（95％ CI：0.555～0.894，P＝0.002）、0.893（95％CI：0.699～0.966，P＝0.037），敏感性分别为75.9％、62.1％、75.9％，特异性分别为90.0％、88.3％、92.1％。结论胃癌患者血清中miR-192和miR-215表达可能与胃癌的形成或发展有关。 miR-192、miR-215检测及其联合应用，对胃癌的诊断有较高的敏感性和特异性，提示这两种血清miRNA有望作为胃癌的诊断标记物。

英文摘要：

Objective To explore the value of miR-192 and miR-215 in the diagnosis of gastric cancer . Methods Sera were collected from twenty nine cases of preoperative gastric cancer patients and ten healthy controls.The clinical data of gastric cancer patients were collected .After extracted the total RNA in the serum samples,expression levels of miR-192 and miR-215 were determined by quantitative reverse transcription polymerase chain reaction（qRT-PCR）,and calculation using the 2-△Ct method for evaluation of the association between miR-192, and miR-215 expression levels and clinic-pathological characteristics .The sensitivity and specificity of diagnosis for gastric cancer were evaluated by using receiver operating characteristic （ ROC） curve,and area under the ROC curve （ AUC） .Results The expression of miR-192 and miR-215 had an 8.87-fold （ P =0.002 ） and 4.14-fold （ P =0.037 ） increase in the sera of gastric cancer patients compared with normal control ,respectively .MiR-192 and miR-215 expression levels were not significant different between different tumor stage ,different lymph node metastasis and different tumor size .The AUC for miR-192 , miR-215 , and combination were 0.833 （ 95%CI：0.793-0.993 , P 〈0.001）,0.724（95%CI：0.555-0.894,P=0.002）,and 0.893（95%CI：0.699-0.966,P=0.037）,respectively.The sensitivity for miR-192 , miR-215 , and combination were 75.9%, 62.1%, 75.9%, and specificity for them were 90.0%,88.3%,92.1%,respectively .Conclusion Altered expression of miR-192 and miR-215 might be involved in the pathogenesis of gastric cancer .The detection of miR-192 and miR-215 in serum might be a novel tool for diagnosis of gastric cancers .