利用分子标记技术分析了山西5个天然油松种群140个个体的遗传特征,比较了不同种群的多态位点显性频率、遗传多样性指数、基因流和分化系数.筛选的5个ISSR引物和5个RAPD引物共扩增到位点68个,其中多态位点50个遗传参数的统计分析显示,多态位点的显性频率、遗传多样性指数、分化系数分别介于0.0000~1.0000、0.0200~0.500O和0.0037~0.6156之间,表明油松种群遗传多样性的主要来源是等位基因频率的不同和部分位点较大程度的遗传分化.根据油松的种群遗传学特征,认为只有加强天然油松林的生境保护,使其不产生片断化,才能使油松的遗传资源得到有效保护.图2表4参16
Pinus tabulaeformis widely distributes in China from northeast to southwest, and plays an important rote in natural forests and artificial plantations. Its genetic characteristics of DNA in 140 samples from 5 populations in Shanxi were investigated by using molecular technique. A total of 68 reproducible bands were identified using 5 RAPD primers and 5 ISSR primers, and 50 bands of them were polymorphicl loci. The statistic analysis of the genetic parameters indicated that gene frequency, genetic diversity index and the relative magnitude of differentiation among populations were 0.000 0-1.000 0, 0.020 0-0.500 0 and 0.003 7-0.615 6, respectively, suggesting that the genetic diversity ofP. tabulaeformis populations in Shanxi mainly lies on different allelic frequencies and on some high level genetic differentiating locus. Based on the characteristics of P. tabulaeformis populations in Shanxi, it is suggested that the genetic resource of pine can only be conserved well by enhancing the protecting ofP. tabulaeformis habitats. Fig 2, Tab 4, Ref 16