中文摘要：

目的：探讨葡萄球菌肠毒素A（staphylococcal enterotoxin A，SEA）对甲磺酸伊马替尼（imatinibmesylate，IM）抑制T淋巴细胞活化的影响。方法：2mg／LSEA和50nmol／LIM联合作用于Jurkat细胞24h后，用实时荧光定量PCR技术检测CD3ε和ζ链mRNA表达水平变化；Westernblotting技术检测CD3ε和ζ链蛋白水平变化。结果：单纯IM组CD3e和链mRNA及蛋白表达均表现下调；SEA与IM联合用药组可以逆转IM下调CD3ε和ζ链mRNA及蛋白表达水平作用。SEA拮抗IM抑制作用中，对CD3e链mRNA表达上调的作用明显大于CD3ζ链。结论：SEA能拮抗IM对T细胞CD3ε和ζ链表达的抑制作用。

英文摘要：

AIM： To investigate the effects of staphylococcal enterotoxin A （SEA） on the inhibition of T lym phocyte activation induced by imatinib mesylate （IM）. METHODS： Jurkat cells were stimulated with SEA （2 mg/L） and IM （5 nmol/L） for 24 h. The mRNA expression of CD3ε and chains was measured by real-time fluorescence quantitative PCR. The protein levels of CD3ε and ζ chains were detected by Western blotting. RESULTS： The expression of CD3ε and ζchains at mRNA and protein levels was down-regulated in the Jurkat cells stimulated by IM alone. These down-regu lations of CD3ε and ζchains were reversed by the stimulation of IM combined with SEA. The antagonistic effect of SEA on IM-mediated inhibition of CD3ε mRNA expression was significantly greater than that on CD3 mRNA. CONCLUSION： SEA antagonizes imatinib-mediated inhibitory effect on T cell activation.