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中文摘要：
定量蛋白质组学已经成为蛋白质组学的一个重要分支,以生物质谱为核心的定量蛋白质组方法日益发展。按照定量所依据的质谱信号来源于一级质谱谱图还是多级质谱谱图可以将定量蛋白质组方法分为一级质谱定量和多级质谱定量。本文主要综述基于多级质谱的定量方法和技术进展,分析比较了这些方法的优缺点,并对基于多级质谱的定量方法发展进行了展望。
英文摘要：
With the development of proteome research,quantitative proteomics is becoming a very active area of proteomics research.The development of quantitative proteomics requires the advancement of a number of mass spectrometry（MS）-based quantitative techniques.According to the mass spectrometric stage at which the peptides are quantified,MS-based quantitative methods can be divided into two categories： MS1-based quantitation and MSn-based quantitation.In this review,the progress of MSn-based quantitation approaches is summarized and the advantages and disadvantages are compared.The trend of MSn-based quantitation is also prospected.
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