中文摘要：

钛二氧化物(TiO ₂) 薄电影在轻设法解决的 potentiometric 传感器(臀部) 的表面上被扔为 DNA 分子的非标记的察觉修改传感器表面。评估效果紫外(紫外) TiO ₂ 的 silanization 水平上的处理由 3-aminopropyltriethoxysilane (APTS ) 的薄电影，荧光黄 isothiocyanate (FITC ) 被用来在使不能调动到 TiO ₂ 上的 APTS 的结束把胺组标记薄电影。我们发现与紫外照耀， TiO ₂ 电影的照耀的区域的 silanization 水平在控制得好的条件下面与非照耀的区域相比被改进。这结果显示 TiO ₂ 能对生物传感器表面作为涂层材料起作用在传感器表面上改进简历分子的共有原子价固定的效果和效率。人工地综合的探查 DNA 分子 covalently 被连接到 TiO ₂ 电影的表面上。探查 DNA 和目标 DNA 的杂交被记录在反应的过程期间沿着电压轴变的 I-V 曲线监视。重要臀部表明的 A 能在目标 DNA 样品的 10 μ m ol/L 被检测。

英文摘要：

Titanium dioxide （TiO2） thin film was deposited on the surface of the light addressable potentiometric sensor （LAPS） to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet （UV） treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane （APTS）, fluorescein isothiocyanate （FITC） was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that, with UV irradiation, the silani- zation level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and effi- ciency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.