中文摘要：

目的检测乙型肝炎病毒X蛋白（HBx）在肝细胞癌中的表达及其与患者预后的关系，并探讨人血清HBx抗原的检测。方法用免疫组化法检测35例肝癌患者肿瘤组织中HBx蛋白的表达，与临床资料进行相关分析；应用酶联免疫吸附法（ELISA）检测77例人血清中HBx抗原的表达情况。结果肝癌组织中HBx的总体阳性检出率为20／35（57．14％），其中乙型肝炎病毒表面抗原（HBsAg）阳性者HBx的阳性率为15／24（62．50％），而HBsAg阴性者HBx的阳性率为5／11（45．45％）；HBx阳性与HBx阴性的肿瘤组织低分化比率分别为9／20（45．00％）及2／15（13．33％），差异有统计学意义（P〈0．01）；HBx阳性肝癌患者的无病生存期（DFS）明显短于HBx阴性患者（Kaplan—Meier法及log—rank检验，P=0．03）。ELISA检测人血清中HBx抗原的总阳性率为48／77（62．34％）。结论肝癌肿瘤组织中HBx的表达与患者无病生存期呈负相关，ELISA法能有效检测人血清中HBx抗原的表达．

英文摘要：

Objective To detect the expression of HBx in HCC and analyze its relationship with disease-free survival （DFS） in HBV-related HCC. To evaluate the detection performance of HBx antigen in human serum. Methods Immunohistoehemical staining was utilized to detect the expression of HBx in 35 HBV-related HCC tissues, and results were analyzed with clinical data. Enzyme-linked immunosorbent assay （ELISA） was applied to detect the HBx antigen in human serum. Results HBx positive ratios were 20/35 （57.14%） in HCCs, with 15/24 （62.50%） of HBsAg positive and 5/11 （45.45%） of HBsAg negative cases respectively. The poorly differentiated ratio of HBx positive （9/ 20, 45.00%） was significantly higher than that of HBx negative （2/15, 13.33%） cases （P〈0.01）. Disease free survival （DFS） of HBx positive and negative group was estimated by Kaplan-Meier method and compared by log-rank test. The results showed poor DFS in HBx positive group （P=0.03）. ELISA could efficiently detect HBx antigen in human serum with 48/77（62.34%） positive. Conclusions The expression of HBx is correlated with tumor pathological grading and poor DFS of HCC patients. ELISA can efficiently detect HBx antigen in human serum.