中文摘要：

目的探讨锌缺乏对增殖期大鼠肋生长板软骨细胞(RGC)凋亡的影响,为阐明锌对骨骼生长发育影响的机制提供科学依据。方法体外培养Wistar大鼠RGC,用0,5,10及20μmol/L的锌螯合剂N-N-N′-N′-4(2-吡啶甲基)乙二胺(TPEN)分别处理细胞12h。采用流式双染试剂盒研究早期凋亡和晚期凋亡细胞百分比。以5μmol/LTPEN作用6,12,24h,利用免疫组化技术检测细胞中凋亡诱导因子(AIF)的定位表达,并利用Western blot和实时定量PCR(real-time PCR)检测其表达水平的变化。结果随着TPEN浓度的增高,RGC细胞凋亡率也逐渐增加,各组早期凋亡和晚期凋亡细胞百分比增大,AIF的表达水平也逐渐升高。免疫组化染色结果显示在TPEN作用6h后,AIF已从线粒体迁出,12h及24h后,大部分AIF已从线粒体迁至细胞核。结论锌缺乏可以诱导RGC细胞凋亡,在凋亡的过程中AIF发挥着关键作用。

英文摘要：

Objective To study the effect of zinc deficiency on the apoptosis of prolierative growth plate chondrocytes and thus provide direct evidence for studying the mechanism of the effect of zinc in bone growth.Methods Growth plate chondrocytes were isolated from Wistar rats and treated with 0,5,10,and 20 μmol/L of TPEN for 12 hours,respectively.The percentages of cells in early and late stage of apoptosis were determined by flow cytometry.Immunohistochemical staining was performed to identify the location of apo...