中文摘要：

目的通过观察锌指结构转录因子Osterix（Osx）在不同染氟模型中的表达,探讨在氟性骨损伤骨周化骨过程中Osx因子的作用。方法采用实时荧光定量RT-PCR的方法检测不同染氟剂量和时间段（实验组氟浓度分别为0.01、0.1、1、10mg/L,对照组为0mg/L）成纤维细胞OsxmRNA的表达水平,Western blot法检测Osx蛋白在染氟成纤维细胞中的表达。结果染氟时间段Osx蛋白呈先升高后下降的趋势,其中在染氟24～48h时的各剂量组Osx蛋白升高趋势比较明显（P〈0.05）;与染氟0h组相比,随着染氟剂量的增加,Osx蛋白总体上也呈先增高后下降的趋势,其中染氟24h和48h升高比较明显（P〈0.05）;RT-PCR结果显示,与对照组相比,OsxmRNA的表达在染氟24～48h的各剂量组明显升高（P〈0.05）,各染氟时间段OsxmRNA的变化和Osx蛋白变化的趋势一致,均呈先升高后下降的趋势。结论氟能促进Osx因子在成纤维细胞中的表达,提示Osx因子可能在氟引起成纤维细胞向成骨细胞转化,进一步引起氟性骨损伤骨周化骨发生发展的过程中起重要作用。

英文摘要：

Objective To observe the expression of zinc finger transcription factor Osterix（Osx） in different groups of floride（F） exposure in order to investigate its role in the cause of fluoride bone injuries and ossification around bone.Methods Western blot and fluorescence real-time quantitative reverse transcription polymerase chain reaction（RT-PCR） were used to detect the expressions of Osx mRNA and its protein in fibroblast,and the concentrations of F was 0.01,0.1,10mg /L in experimental groups,and the control was 0 mg /L.Results The levels of Osx protein in F exposure groups increased at first and then decreased with the levels of F exposure,in which a significant increases were found after fibroblasts were exposed to F for about 24-48h in all experimental groups（P 0.05）.In comparition with the 0h F exposure group,the trend of Osx was the same,the results of RTPCR indicated that the levels of Osx mRNA significantly increased in the group of 24-48h F exposure groups（P 0.05）,overall,the trends of Osx mRNA level were similar to the trend of Osx protein,which went up at first and then went down in each group.Conclusion The expressions of Osx factor in fibroblast could be promoted by fluoride,which could play an important role in the process of the transformation from fibroblasts to osteoblast induced by fluoride,and in the development of fluoride bone injuries and ossification around bone.