中文摘要：

目的对5省份屠宰场鸡胴体中分离的空肠弯曲菌进行鉴定和分子分型，为建立空肠弯曲菌食物中毒的追踪溯源数据库提供科学数据。方法采用生化和分子生物学方法筛检和鉴定鸡专项监测网报送的鸡胴体中分离株。利用属特异16SrRNA基因和种特异的MapA、CeuE基因引物，建立多重PCR反应，鉴定空肠弯曲菌；参照国际认可的PFGE方法，选用内切酶SmaI和KpnI分别进行单酶切，并利用BioNumerics软件对分离株的电泳指纹图谱进行聚类分析，所得结果收入溯源数据库。结果对81株屠宰场鸡胴体分离株进行生化和PCR鉴定，确证72株为空肠弯曲菌，9株为结肠弯曲菌；脉冲场凝胶电泳分子分型结果表明，这72株空肠弯曲菌共产生48种带型。通过聚类分析，按照63．9％相似度将这些菌株分为A～M共13个群。每群包含1～11个带型，72株菌的带型分布具有完全地域同源性，即同一带型的菌株均来自同一省份。结论采用SmaI和KpnI单酶切进行PFGE分型，结合聚类分析，可提高PFGE分型的分辨率，增加菌株分型的准确性和溯源的可信性。

英文摘要：

Objective To characterize and investigate the molecular types of Campylobacter jejuni isolated from slaughter chicken carcass, which would provide scientific data for campylobacter food poisoning traceability. Methods Biochemical and molecular biological methods were used for screening and identification of isolates from chicken special monitoring networks. Campylobacter Genus-specific primers 16S rRNA and species-specific primers MapA and CeuE were designed to perform a multiplex PCR to identify these isolated strains. Pulsed-field gel eleetrophoresis （PFGE） was employed to type Campylobacter jejuni isolates by digesting with restriction endonuelease Sma I and Kpn I respectively. Fingerprints of these isolates were analyzed by the software BioNumeries. Results 72 out of 81 isolates were confirmed as Campylobacter jejuni by biochemical test combined with PCR. 48 patterns were obtained from PFGE with Sma I and Kpn I . 72 isolated strains were divided into 13 clusters （A-M） according to 63.9% similarity by cluster analysis. Isolates from different provinces were distributed in 13 clusters and each cluster contained 1 to 11 patterns. The results showed that the 72 strain patterns distribution had complete regional homology, namely strains in the same pattern were from a single province. Conclusion The comprehensive analysis of Sma I and Kpn I results may help improving the resolution of PFGE reliability of traceability. , increasing the accuracy of typing and