中文摘要：

目的 ：建立简单快速的LC-MS/MS检测大鼠血浆中紫杉醇浓度的方法。方法：血浆样本100μL加入终浓度为100ng/mL的多烯紫杉醇（内标），用混合溶剂乙酸乙酯-二氯甲烷-乙腈（4：1：1）萃取后用LC-MS/MS进行分析。采用多离子反应监测（MRM）扫描方式，在ESI源正离子加钠模式下，紫杉醇和内标物的离子对分别为m/z 876.29→308.1、m/z830.34→549.3。结果：样品分析时间仅需2min，方法线性范围在0.55-550ng/mL（r=0.997），最低检测限为0.55ng/mL。紫杉醇在三个质控浓度（1.1, 110, 550ng/mL）检测下的提取回收率分别为79.32%，77.54 %及78.64%。结论：LC-MS/MS检测方法灵敏度高、专属性强、简单快捷，采用加钠模式可增加紫杉醇检测的响应值，提高检测灵敏度，适用于检测血浆中微量紫杉醇的浓度。

英文摘要：

OBJECTIVE To establish a simple and rapid LC-MS/MS method for the determination of paclitaxei in rat plas ma. METHODS He 100 bd. of sample plasma was extracted with mixed solvent （ethyl acetate：dichloromethane： acetonitrile = 4 ： 1 ：.1） after addition of 100 ng,mL^-1 doeetaxel（internal standard）, and analyzed by LC-MS/MS system. Detection was per- formed with multiple reactions monitoring （MRM） using electrospray ionization （ES1） and adding Na ion mode. The precur- sor/product ion transitions of paclitaxel and docetaxel（internal standard）were monitored at m/z 876. 29→308. 1 and 830.34± 549. 3, respectively. RESULTS The analysis time was only 2 rain in positive mode; the calibration curve was linear in the con- centration range of 0. 55-550 ng·mL^-1. The lowest limit of quantification （LLOQ） reached 0. 55 ng·mL 1. The extraction recoveries were 79.320/00, 77. 54 % and 78. 64% for three QC concentration levels （1.1, 110, 550 ng·mL ^-1）. CONCLU- SlONThe I.C-MS/MS method proved to be sensitive, specific, simple and rapid; using the adding sodium mode can increase pa- clitaxel detection response value, improve the detection sensitivity, suitable for detection of trace paclitaxel in plasma.