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亮叶忍冬组织培养快繁技术
  • ISSN号:1001-0009
  • 期刊名称:《北方园艺》
  • 时间:0
  • 分类:S793.9[农业科学—林木遗传育种;农业科学—林学]
  • 作者机构:[1]天津农学院园艺系,天津300384
  • 相关基金:国家科技星火计划资助项目(2008GA610015);天津市科委资助项目(08ZHXHNC07000).
中文摘要:

分别采用添加不同浓度6-BA的MS培养基和不含植物激素但改变大量元素浓度的MS培养基2种方法,研究亮叶忍冬组培苗快繁体系。结果表明:以不含激素的1/4Ms培养基中亮叶忍冬的茎芽增殖效果最好,增殖系数达3.8。将亮叶忍冬试管苗茎段培养于用土做支撑物的培养基中,生根率为98.3%,根数也与琼脂支撑培养对照无明显差异,且根毛长于对照。对土支撑培养基中生根的亮叶忍冬试管苗开瓶练苗3周后,带坨移入营养钵中,在移栽后不喷雾、不覆膜,温室内午后空气相对湿度低至55%的条件下,移栽成活率达到93.3%,显著高于常规方法移栽的成活率(53.3%)。

英文摘要:

This paper aims at setting up the system of tissue culture and rapid propagation technique of Lonicera nitida ' Maigrun'. The MS media containing different concentration of 6-BA and the MS media with major element concentration changed without plant hormone were used respectively for the micro shut multiplication. The micro shuts multiplied best with the multiple coefficient of 3.8 on the 1/4 MS medium without plant hormones. The micro stem sections of Lonicera nitida 'Maigrun' were cultured in soil supporting medium and 98. 3% of them rooted,the root number of which was no significant difference comparing with that of cultured in medium supported by agar,the root hairs of the plantlet from soil supporting medium were longer than that from agar supporting medium. After acclimatized in opened bottles for three weeks the rooted plantlets in soil supporting medium were transplanted into soil with lump and under the conditions of no-spraying, no-covering and low air humidity of 55 % in afternoon their survival rate was 93. 3 % which was significantly higher than that of the plantlet transplanted by normal method,the latter survival rate was 53. 3%.

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期刊信息
  • 《北方园艺》
  • 北大核心期刊(2011版)
  • 主管单位:黑龙江省农科院
  • 主办单位:黑龙江省园艺学会 黑龙江省农业科学院
  • 主编:毕洪文
  • 地址:哈尔滨市南岗区学府路368号
  • 邮编:150086
  • 邮箱:bfyybjb@163.com
  • 电话:0451-86674276
  • 国际标准刊号:ISSN:1001-0009
  • 国内统一刊号:ISSN:23-1247/S
  • 邮发代号:14-150
  • 获奖情况:
  • 1996年获黑龙江省优秀期刊奖(二等),2000年获黑龙江省优秀期刊奖,2002年获全国优秀农业期刊奖,2005年获首届黑龙江省出版精品工程提名奖,2009年获中国北方优秀期刊奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:36972