位置:成果数据库 > 期刊 > 期刊详情页
柑橘种间体细胞杂种的核质遗传研究
  • 期刊名称:果树学报
  • 时间:0
  • 页码:183-187
  • 语言:中文
  • 分类:S666[农业科学—果树学;农业科学—园艺学]
  • 作者机构:[1]扬州大学园艺与植物保护学院,江苏扬州225009, [2]华中农业大学.作物遗传改良国家重点实验室,武汉430070
  • 相关基金:国家自然科学基金(编号:30200189)
  • 相关项目:柑桔原生质体全能性表达及其受阻机理研究
中文摘要:

为了解柑橘体细胞杂种的核质遗传组成,采用流式细胞仪(FCM)、简单序列重复(SSR)、相关序列扩增多态性(SRAP)以及酶切扩增多态性序列(CAPS)对此前通过对称融合获得的2株种间体细胞杂种进行了分析。结果表明,在丹西红橘+红江橙种间组合中,分析的2株植株中1株为二倍体非对称体细胞杂种,其核DNA具有双亲的部分DNA,并有重组发生,胞质DNA则来源于红江橙(叶肉亲本);而另1株则是异源四倍体体细胞杂种,其核DNA来源于双亲,并有重组发生,胞质DNA则来源于红江橙。

英文摘要:

FCM,SSR,SRAP and CAPS were employed to characterize the nuclear and cytoplasmic components of two inter-specific somatic hybrid plants obtained by symmetric fusion.The results showed that in Dancy tangerine(Citrus reticulata)+ sweet orange(C.sinensis Osbeck cv.Hongjiangcheng) inter-specific combination,one of two analyzed plants was diploid asymmetric somatic hybrid,which inherited nuclear DNA from some of both parents and cytoplasmic composition from sweet orange(leaf parent);the another one was tetraploid heterokaryonic somatic hybrid,which inherited nuclear DNA from both parents and cytoplasmic composition from sweet orange(leaf parent),and recombination of nuclear DNA occurred in the two plants.

同期刊论文项目
同项目期刊论文