中文摘要：

基于血清代谢组学和定量差异探讨2型DM大鼠的运动干预机制。Sprague dawley大鼠分为正常对照组（NC）、DM模型组（DMC）和DM运动干预组（DME）。DMC和DME组大鼠予以高糖高脂饲料喂养4周,以40 mg/kg腹腔注射链脲佐菌素造模稳定1周。DME组大鼠实施游泳运动干预8周。采用超高效液相色谱-四级杆飞行时间质谱联用仪（UPLC/Q-TOF MS）技术对不同组别大鼠的血清进行代谢组学分析,利用定量差异对代谢组学获得的生物标记物进行筛选。结果显示：1）DME组、DMC组和NC组的血清代谢谱明显分离,表明DME组血清代谢谱与DMC组和NC组之间有显著的变化;2）血清中精氨酸、脯氨酸-甜菜碱、二十碳五烯酸、亚麻酸、丙基肉碱、单甘油酸酯（24：6）、肉毒碱、1-磷酸鞘氨醇、葡糖酸、和磷酸胆碱（20：3）共10种代谢产物在DMC组和NC组间存在显著性定量差异（l〉0.80）,除了单甘油酸酯（24：6）和磷酸胆碱（20：3）外,8周游泳训练可以将DME大鼠体内的其它8种物质恢复到与NC组没有显著性差异（l〈0.47）。结果表明：（1）游泳训练可以完全康复2型DM大鼠。（2）多不饱和脂肪酸和精氨酸代谢有望成为今后研究运动干预2型DM机制的新鲜靶点。

英文摘要：

In order to probed into the mechanism of exercise intervention on type 2 diabetes mellitus （DM） rats based on serum metabonomics and quantitative difference, the authors divided Sprague Dawley rats into a normal control group （NC）, a DM control group （DMC） and a DM exercise group （DME）, fed the rats in groups DMC and DME with high-sugar high-fat feed for 4 weeks, and intraperitoneally injected them with streptozotocin at a dose of 40 mg/kg body weight for 1 week in order to stabilize model establishment, implemented swimming intervention on the rats in group DME for 8 weeks, carried out a metabonomic analysis on the serum of the rats in different groups by using ultra performance liquid chromatography coupled with time-of-flight mass spectrometry （UPLC/Q-TOF MS）, screened biomarkers acquired from the metabonomic analysis by utilizing quantitative difference, and revealed the following findings： 1） there was a clear separation of serum metabolic profile of the rats in groups DME, DMC and NC, indicat- ing that there was a significant change in serum metabolic profile between the rats in group DME and the rats in groups DMC and NC; 2） between the rats in groups DMC and NC, there was a significant quantitative difference in Arginine, Proline betaine, Eicosapentaenoic acid, Linolenic acid, Propionyl-L-carnitine, MG（24：6）, Carnitine, Sphingos-ine-1-phosphate, Gluconic acid and PE（20：3）（l〉0.80）, except MG（24：6） and PE（20：3） were completely recovered after 8-week exercise intervention（l〈0.47）. The said findings indicate the followings： 1） swimming training can fully rehabilitate type 2 DM rats; 2） polyunsaturated fatty acid and arginine metabolism are hopefully to become new targets of study of the mechanism of exercise intervention on type 2 DM in the future.