比较了两种土样制备和保存方法对厌氧培养1周内土壤反硝化及矿化的动态影响。试验结果表明,强烈风干后并经长期存放过的土样显著促进了NO3^--N浓度降低速率和N20排放速率的提高,其反硝化速率和矿化速率分别较稍微风干后无存放时间(即立即开始培养试验)的土样提高了47.3%和31.0%。强烈风干土有机C矿化作用的增强以及易矿化有效态C含量的提高是促进反硝化作用增强的主要原因。风干程度和存放时间对反硝化的促进程度取决于其对有机质矿化影响的相对大小,对有机质矿化的影响越大,反硝化强度增加的幅度也越大。由试验结果可推测,利用风干土的实验室培养方法测定得到的土壤反硝化势可能会过高估计田间原位测定的反硝化势。
Two soil sample treatments, which were less dramatically air-dried and immediately commenced incubation experiment (treatment 1 ) and more dramatically air-dried and stored for a long time (treatment 2), were used in the experiment. Effects of the two methods of soil samples on denitrification and mineralization were compared during a 7d-anaerobic-incubation. The results suggested that soil samples under treatment 2 significantly stimulated the decrease of NO3^- content and the increase of N2O emission rate. Denitrification rate and mineralization rate in soil samples under treatment 2 were 47.3% and 31.0% higher than those of the soils under treatment 1, respectively. Air-drying enhanced soil organic matter mineralization and increased the availability of organic carbon, which was the main reason for the higher denitrification capacity in more dramatically air-dried soils. The enhanced extent of denitrification by the air-drying degree and the storage period in air-dried soils was determined by the promoted extent of organic matter mineralization. The more organic matter mineralized, the more NO3 denitrified. Therefore, the results deduced from the incubation experiment with alr-dried soil samples may overestimate in situ denitrification capacity.