田间采集的新鲜土壤样品分别在室温下风干(土样D)和淹水(土样S)保存110d后,将二者的含水量分别调至20%、40%、60%、80%、100%持水量(WHC,Water Holding Capacity),在25℃下培育138h,设置不通和通人10%(v/v)乙炔的处理。结果显示,在20%~80%WHC下培育时,土样S的氧化亚氮(N2O)排放量为土样D的2.48倍~6.36倍(P〈0.01),而在100%WHC水分含量下培育时,土样S的N2O排放量仅为土样D的19%(P〈0.01),通人乙炔不但未使土样D的N2O排放量增加,反而显著减少。通入乙炔的处理,培养结束后硝态氮的含量增加。随培育水分含量的升高,土样S和土样D的二氧化碳排放量增大。供试土样可能存在异养硝化作用。前期水分的差异显著影响土壤N2O排放量,故在田间测定土壤N2O排放量时,要考虑土壤前期水分的差异。
A laboratory incubation experiment was conducted to investigate nitrous oxide ( N2O) emission from an arable Uhisol as affected by antecedent water regime. The soil was maintained either air-dried (Sample D) or submerged (Sample S) for 110 days before the two soil samples were adjusted in soil moisture up to or down to 20% , 40% , 60% , 80% and 100% water holding capacity (WHC) , respectively. And then they were incubated with or without 10% (v/v) acetylene for 138 h at 25℃. Results show N2O emission from Sample S was 2.48 to 6.36 times as much as that from Sample D with soil moisture content ranging from 20% to 80% WHC (p 〈0. 01 ). When the soil moisture content was adjusted to 100% WHC, N2O emission from Sample S was only 19 percent of that from Sample D, and N2O emission from Sample D incubated with acetylene was lower than that without acetylene (p 〈 0.01 ). The content of nitrate increased after incubation with acetylene. CO2 emission from Samples S and D increased with the soil moisture content. Heterotrophie nitrification might be present in the tested soil. Antecedent water regime significantly influences N2O emission, and should hence be considered as a factor in determination of N2O emission in situ.