中文摘要：

目的探讨纤维连接蛋白连接片段-1（CS1）肽段对大鼠肝移植缺血再灌注损伤的影响及其机制。方法用Wistar大鼠作为供、受鼠，制作肝移植模型。CS1组供鼠分别于术前3d、取肝时和移植前注射CS1肽段，供肝获取后于uw液中保存18h，肝移植术后3d每天注射CS1肽段；对照组用随机肽段替代CS1，其他操作同csl组。术后6、24、72h检测受鼠血清转氨酶水平和肝组织病理改变。免疫组织化学染色显示肝脏中的炎症细胞和肝窦内皮细胞。多聚酶链反应检测肝组织中肿瘤坏死因子-a（TNF-a）、白细胞介素-1p（IL-1β）和血管内皮细胞生长因子（VEGF）mRNA的表达水平。结果术后24h，对照组坏死肝组织面积约占总面积的（25．7±5．4）％，而CS1组为（12．6±3．6）％（P〈0．05）。对照组血清转氨酶水平也低于对照组（P〈0．05）。术后CS1组移植肝中枯否细胞和中性粒细胞数目均少于对照组（P〈0．05）。两组冷缺血18h的供肝中，肝窦内皮细胞均失去正常形态，仅少数内皮细胞特异性标志物阳性。术后72h，CSI组肝窦内皮细胞基本恢复正常形态，SE-1表达恢复，而对照组肝窦内皮细胞恢复欠佳。术后6和24h时，CS1组肝组织中TNF-amRNA的水平低于对照组（P〈0．05）；术后24h时，对照组VEGFmRNA的表达高于CS1组（P〈0．05）；两组IL-1βmRNA水平的差异无统计学意义（P〉0．05）。结论用CS1肽段处理供、受鼠可以减少炎症因子mRNA的表达，保护肝窦内皮细胞，减轻移植肝缺血再灌注损伤。

英文摘要：

Objective To examine the effect of fibronectin connecting segment-1 （CS1） peptidefacilitated blockade of inflammatory cells-fibronectin adhesion on a rat liver transplantation model of prolonged ex vivo cold ischemia. Methods A model of liver transplantation in Wistar-Wistar rat was established. The donors of the CS1 treatment group received CS1 peptides through the tail vein for 3 days before operation. Another two doses of CS1 peptides were administered into the liver intraportally during procurement and before transplantion. Recipients received an additional 3-day course of CS1 peptides after transplantation. Rats in control group received scrambled peptides. Rats were sacrificed at 6, 24 and 72 h after transplantion, and plasma transaminase activity and hepatic pathological changes were studied. The inflammatory cells and liver sinusoidal endothelial cells were visualized histochemically. Real-time PCR was used to detect tumor necrosis factor-a （TNF-a）, interleukin-lβ （IL-1β） and vascular endothelial growth factor （VEGF） mRNA expression in the liver. Results The plasma transaminase activity and hepatic necrosis areas in CS1 treatment group were significantly lower than in control group （P〈0. 05）. CS1 peptides treatment significantly decreased the number of Kupffer cells after transplantation and greatly inhibited the recruitment of neutrophils to the graft liver as compared with control group （P〈0. 05）. After prolonged cold isehemia, only a few hepatic end6thelial cells exhibited positive staining of hepatic sinusoidal endothelial cell biomarker SE-1. Lots of hepatic sinusoidal endothelial cells positive for SE-1 staining could be detected in CS1group at 72 h after transplantation, while much less SE-1 positive ceils presented in the control goup. Prolonged cold ischemia caused a significant increase of TNF-a, IL-1β and VEGF mRNA expression in the graft liver of control group after transplantation. The expression of TNF-a mRNA at 6 and 24 h and VEGF mRNA expression at 24 h were