中文摘要：

植物寄生性线虫类FMRF酰胺多肽（FMRFamide-like neuropeptides,FLPs）在线虫的取食、分泌、迁移入侵和繁殖过程中发挥着重要的作用。本研究根据EST序列结合RACE方法,获得了一个马铃薯腐烂茎线虫（Ditylenchus destructor）类FMRF酰胺多肽基因的cDNA全长。该基因cDNA全长序列为891bp,包括一个681bp的完整开放阅读框,编码一个包含227个氨基酸的蛋白,预测的分子量为25.36kD。序列分析表明,该蛋白N端有一个由43个氨基酸残基组成的信号肽,C端含117个氨基酸的FMRF酰胺肽的特征区,其中包含有5个NFLRFG的FLPs肽,属于典型的FLP-1型蛋白,该基因命名为Dd-flp-1（GenBank登录号为GU198750）。系统发育分析表明,Dd-FLP-1与自由生活线虫、动物寄生线虫和其他迁移性植物寄生线虫的FLPs属于同一支,推测线虫FLP的进化与其生活习性密切相关。原位杂交结果表明,Dd-flp-1在马铃薯腐烂茎线虫的咽神经环、后膀胱神经节多个细胞特异表达。推测DD-FLP-1主要与马铃薯腐烂茎线虫的取食、运动和排泄功能相关。本研究结果将为植物线虫flp基因功能的研究和寻求新的植物寄生线虫防治策略提供理论依据。

英文摘要：

FMRFamide-like peptides （FLPs） play an important role in infection, feeding and reproduction of plant parasitic nematode. A novel FMRFamide-like neuropeptide gene was isolated from Ditylenchus destructor by EST analysis and RACE methods. The full length cDNA of Dd-flp-1 consisted of 891 bp, with a 681 bp ORF encoding a 227 amino acid protein and the predicted molecular weight was 25.36 kD. The protein included a signal peptide of 43 amino acids on the N- terminal and a predicted FMRF domain with five FLPs as NFLRFG on the C- terminal. It was a typical FLP-1-type protein, named Dd-flp-1（GenBank accession No： GU198750）. Phylogenic analysis suggested that Dd-FLP-1 had a homology to free live nematodes, animal parasitism nematodes and othermigratory plant-parasitic nematodes which might indicate association of the evolution of flp genes from plant parasitism nematodes and their habits. In situ hybridization analysis showed that the transcripts of Dd-flp-1 accumulated exclusively within the circumpharyngeal nerve ring, retrovesicular ganglion and ventral cord of D. destructor. The results suggested that the major functions of DD-FLP-1 were closed with feeding, moving and excreting in D.destructor. This study will provide the foundation and theoretical basis for research on the flp gene function and developing novel plant parasite control targets by RNAi