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中文摘要：
β-1,4内切葡聚糖酶是植物寄生线虫食道腺分泌的一类细胞壁降解酶,在植物线虫的侵染过程中具有重要的作用。以马铃薯腐烂茎线虫（Ditylenchus destructor）为材料,用RT-PCR和RACE方法,获得了β-1,4-内切葡聚糖酶基因的cDNA全长,并将该基因命名为Dd-eng-1b（GenBank登录号为FJ430142）。此cDNA全长序列为1640bp,包括1个1443bp的完整ORF,编码1含481个氨基酸的蛋白,其理论分子量为50.89kD,等电点pI为6.94。序列比对分析表明,含有糖基水解酶的保守结构域,属于纤维素酶第五家族成员,N端具有19个氨基酸残基组成的信号肽,C端含有细菌式样的纤维素结合域（CBDⅡ）。cDNA与基因组DNA重叠分析表明,此基因包含4个内含子,长度分别为36、76、187和344bp,切割点符合5＇-GT……AG-3＇的规律。系统进化树分析表明,该基因与细菌Bacillus subtilis和Erwina carotovora分泌的纤维素酶属于同一支,推测该基因可能来源于细菌的水平基因转移。
英文摘要：
β-1, 4-endoglucanase secretions from the oesophageal glands of plant-parasitic nematodes play important roles in infected plant. A new β-1, 4-endoglucanase gene, named Dd-eng-1b, was isolated from Ditylenchus destructor by RT-PCR and RACE. The full length cDNA of Dd-eng-1b consisted of 1 640 bp with a 1 443 bp ORF encoding a 481 amino acid protein （GenBank accession No. FJ430142）. The putative protein with theoretic molecular weight was 50.89 kD and pI was 6.94. The sequence analysis indicated that this gene could be classified as a member of cellulose （glycosyl hydrolase family 5, GHF5） and had a 19 amino acid signal-sequence at N terminal and a high similarity to a bacterial type cellulose-binding domain Ⅱ at C terminal. Comparative analysis between cDNA and genomic showed that Dd-eng-1b contained four introns which length were 36, 76, 187 and 344 bp, respectively, demarcated by 5＇-GT AG-Y in the slpice sites, and phylogenic analysis suggested that Dd-eng-1b had a strong homology to Bacillus subtilis and Etwina carotovora, which may be indicative of an ancient horizontal gene transfer from bacteria.
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