中文摘要：

从长期受对硝基酚（para-nitrophenol,PNP）污染的土壤中,分离到一株能够降解对硝基酚并以其作为唯一碳源和氮源生长的菌株,命名为PN-1。经16S r DNA序列分析,初步鉴定该菌株为假单胞菌（Pseudomona）。通过研究其对PNP的降解、重金属以及抗生素抗性、植物促生等特性,得到如下结果：该菌株对PNP的最大耐受质量浓度为200 mg/L,具有卡那霉素和氯霉素抗性,对4种重金属Cd2＋、Pb2＋、Zn2＋和Cr6＋的最低抑制浓度（minimum inhibitory concentration,MIC）分别为700、500、800和100 mg/L,同时具有产吲哚乙酸（IAA）和铁载体、1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性以及溶磷能力。通过用荧光蛋白标记后能在土壤中正常存活,具有能与植物联合修复PNP污染土壤的潜力,有较好的使用前景。

英文摘要：

A para-nitrophenol（PNP） degrading strain, named PN-1, was isolated from the PNP-contaminated soil. PN-1 utilized PNP as the sole carbon and nitrogen source during the cultivation. Based on the result of 16 S r DNA gene sequence, the strain was preliminarily designated as Pseudomona. Experiments were carried out to investigate the degradation of PNP, heavy metal, antibiotic, plant growthpromoting（PGP） properties and GFP-tagging of PN-1. The results showed that the maximum tolerant dose of TM to PNP was 200 mg/L,and the MICs for the following four heavy metals were 700 mg/L for Cd2 ＋, 500 mg/L for Pb2 ＋, 800 mg/L for Zn2 ＋, and 100 mg/L for Cr6 ＋.What is more, PN-1 could grow in media with chloromycetin and ampicillin. PGP traits of PN-1 included IAA, siderophore production,ACC deaminase activity and phosphate solubilizing activity. GFP-tagged PN-1 could survive and bioremediate PNP-contaminated soil with plants.