中文摘要：

目的探讨乙肝病毒（hepatitis B virus,HBV）感染患者外周血中几种细胞因子在疾病慢性化及疾病进展中的作用。方法分离HBV慢性感染者及健康人外周血,采用酶联免疫吸附法（ELISA法）检测IL-17、IL-23、TGF-β和IL-10的表达及其与乙肝患者肝功能指标及血清中病毒载量的关系。结果乙肝患者血清中IL-17、IL-23和TGF-β与健康对照比较无明显差异,但IL-10显著增加,IL-17、IL-10与转氨酶水平正相关,TGF-β与转氨酶水平负相关,而IL-10与乙肝病毒DNA载量正相关。结论IL-17、IL-10及TGF-β与HBV感染者肝细胞的炎性损伤有关,对于乙肝患者肝纤维化及疾病进展程度具有提示意义,而IL-10具有促炎和耐受的双重作用,可以预测乙肝感染慢性化及病毒持续感染。

英文摘要：

This study aims to explore the level of cytokines and their function in serum of patients of hepatitis B.The levels of IL-17,IL-23,TGF-β and IL-10 were detected by ELISA,while HBV-DNA was detected by RT-PCR.We also tested the liver function of patients.We found that IL-10 was significantly increased in hepatitis B as compared with health control,but IL-7,IL-10 and TGF-β demonstrate no significant difference in the two groups;we also found that IL-17 and IL-10 were positively correlated with transaminase level,TGF-β was negatively correlated with transaminase level,and IL-10 was positively correlated with HBV-DNA.All this result indicate that IL-17,IL-10 and TGF-β are associated with the progress and development of hepatitis B,and IL-10 is associated with the pathogenesis of chronic hepatitis.