中文摘要：

为获得简便高效的提取酵母基因组DNA的方法,分别采用溶菌酶法、蜗牛酶过夜处理法、蜗牛酶反复冻融法和珠磨法处理酵母细胞,在荧光倒置显微镜下观察、比较细胞的破壁情况,提取基因组后利用紫外分光光度计测定样品中DNA的浓度和纯度以及琼脂糖凝胶电泳和PCR扩增检测基因组DNA的质量。结果表明,除溶菌酶法外,其他3种方法均能提取出高质量的酵母基因组DNA。珠磨法提取酵母基因组具有质量高、成本低、时间短且操作简单的优点。

英文摘要：

In order to screen a simple and effective method for the extraction of yeast genomic DNA,different methods,such as lysozyme digestion,overnight snailase digestion,snailase digestion followed by repeated freezing and thawing and grinding with glass beads were compared for their performance based on cell disruption observed under inverted fluorescence microscope,DNA concentration and purity determined using UV spectrophotometer and DNA quality detected by agarose gel electrophoresis and PCR.Except lysozyme digestion,all other methods provided high-quality yeast genomic DNA.The method of grinding with glass beads presented the advantages of high quality extracts,lower cost,less time consuming and ease of operation.