中文摘要：

目的建立基于细胞学水平的循环肿瘤细胞负性筛选方法，检测食管鳞癌患者外周血循环肿瘤细胞。方法选用负性筛选策略，分步去除7．5ml食管鳞癌外周血样本中的红、白细胞，富集上皮来源的稀有细胞。比较3种免疫磁珠（Miltenyi、AVIVA和Xinzhi磁珠）去除白细胞、富集外周血循环肿瘤细胞的特点和效率。用经典的细胞化学染色鉴定循环肿瘤细胞。结果Miltenyi和AVIVA磁珠去除白细胞、富集肿瘤细胞效果较好。从富集后的食管鳞癌患者外周血稀有细胞中鉴定到形态各异的循环肿瘤细胞。结论负性筛选策略可以有效分离鉴定食管鳞癌患者外周血形态各异的循环肿瘤细胞。

英文摘要：

To establish a cell level - based negative selection system to detect circulating tumor cells （CTCs） in the peripheral blood of patients with esophageal squamous cell carcinoma （ESCC）. Methods By Using negative selection system, we depleted red blood cells （RBCs） and white blood cells （WBCs） and enriched the rare cells from 7. 5 ml ESCC patients＇ peripheral blood. Three types of magnetic beads （Mihenyi, AVIVA, and Xinzhi beads） were compared in terms of features and efficiency of WBC＇s depletion and CTCs＇ enrichment. Classic cyto-chemical staining was adopted to identify CTCs. Results Mihenyi and AVIVA beads depleted WBCs and enriched CTCs at high efficiency. CTCs with various morphologies were identified from the enriched rare cells in ESCC patients＇ peripheral blood. Conclusion The cell level-based negative selection system can efficiently isolate and identify CTCs with different morphologies from ESCC patients＇ peripheral blood.