中文摘要：

Poly (D， L-lactide-co-glycolide )(PLGA ) nanoparticles 与 lamivudine 装载了并且与牛的浆液白朊(BSA ) 涂经由一个双乳剂方法被准备。象内部水的阶段，器官的阶段的集中和粒子尺寸和药陷阱效率上的 ultrasonication 时间的体积那样的实验参数的影响被调查，获得有 260 nm 的一条直径和 35% 的药陷阱效率的 PLGA 粒子。粒子被扫描电子显微镜学和发射度电子显微镜学观察，显示出核心壳结构。发现那 58 mg BSA 的 BCA 试金在 1 个 g LPB 粒子上 / 在是在场的。装载 lamivudine 在开始显示出一个爆炸版本并且处于生理的条件支撑了版本直到 24 h。低 pH 能从 PLGA 粒子加速 lamivudine 的版本，使 PLGA 粒子成为潜在的聪明的细胞内部的药搬运人。PLGA 粒子乐意地在一短时间以内被使内在化进人的肝房间并且不管装载 lamivudine 与孵化时间的延伸逐渐地增加了。粒子任何一个在 lysosomes 以内住了或转了到细胞质，但是不能进入房间原子核。房间生存能力显著地没不管 lamivudine 封装面对粒子被影响，建议这种粒子可以是细胞内部的反肝炎 B 药交货的一个好候选人。

英文摘要：

Poly（D,L-lactide-co-glycolide） （PLGA） nanoparticles loaded with lamivudine and coated with bovine serum albumin （BSA） were prepared via a double emulsion method. The influences of experiments parameters such as volume of inner aqueous phase, con- centration of organic phase and ultrasonication time on the particle size and drug entrapment efficiency were investigated, obtain- ing PLGA particles with a diameter of ~260 nm and drug entrapment efficiency of -35%. The particles were observed by scan- ning electron microscopy and transmittance electron microscopy, showing a core-shell structure. BCA assay found that 58 mg BSA was present on/in 1 g LPB particles. The loaded lamivudine showed a burst release at beginning and sustained release until 24 h in physiological conditions. Low pH could accelerate the release of lamivudine from PLGA particles, making the PLGA particles potential intelligent intracellular drug carriers. The PLGA particles were readily internalized into the human liver cells within a short time and increased gradually with the prolongation of incubation time regardless of the loading of lamivudine. The particles either resided within lysosomes or transferred to cytoplasm, but could not enter into the cell nucleus. The cell viability was not significantly influenced in the presence of the particles regardless of lamivudine encapsulation, suggesting that this kind of particles may be a good candidate for the intracellular anti-hepatitis B drug delivery.